Matches in SemOpenAlex for { <https://semopenalex.org/work/W2021637685> ?p ?o ?g. }
Showing items 1 to 100 of
100
with 100 items per page.
- W2021637685 endingPage "192" @default.
- W2021637685 startingPage "183" @default.
- W2021637685 abstract "In this study, we have applied and evaluated a modified cDNA representational difference analysis (RDA) protocol based on magnetic bead technology to study the molecular effects of a candidate drug (N,N'-diacetyl-L-cystine, DiNAC) in a model for atherosclerosis. Alterations in a gene expression profile induced by DiNAC were investigated in a human monocytic cell line (THP-1) differentiated into macrophage-like cells by lipopolysaccharide and further exposed to DiNAC. Three rounds of subtraction have been performed and the difference products from the second and third rounds have been characterized in detail by analysis of over 1000 gene sequences. Two protocols for analysis of the subtraction products have been evaluated, a shotgun approach and size selection of both distinct fragments and band-patterned smear. We demonstrate that in order to obtain a representative view of the most abundant gene fragments, the shotgun procedure is preferred. The obtained sequences were analyzed against the UniGene and Expressed Gene Anatomy Database (EGAD) databases and the results were visualized and analyzed with the ExProView software enabling rapid pair-wise comparison and identification of individual genes or functional groups of genes with altered expression levels. The identified differentially expressed gene sequences were comprised of both genes with known involvement in atherosclerosis or cholesterol biosynthesis and genes previously not implicated in these processes. The applicability of a solid-phase shotgun RDA protocol, combined with virtual chip monitoring, results in new starting points for characterization of novel candidate drugs." @default.
- W2021637685 created "2016-06-24" @default.
- W2021637685 creator A5004126862 @default.
- W2021637685 creator A5036302804 @default.
- W2021637685 creator A5054656710 @default.
- W2021637685 creator A5070016214 @default.
- W2021637685 creator A5080838497 @default.
- W2021637685 creator A5089553620 @default.
- W2021637685 date "2001-06-01" @default.
- W2021637685 modified "2023-10-02" @default.
- W2021637685 title "Monitoring of the subtraction process in solid-phase representational difference analysis: characterization of a candidate drug" @default.
- W2021637685 cites W179805595 @default.
- W2021637685 cites W1959807433 @default.
- W2021637685 cites W1970156673 @default.
- W2021637685 cites W1975029578 @default.
- W2021637685 cites W1978368163 @default.
- W2021637685 cites W2012617920 @default.
- W2021637685 cites W2025054558 @default.
- W2021637685 cites W2026439488 @default.
- W2021637685 cites W2027590165 @default.
- W2021637685 cites W2028288720 @default.
- W2021637685 cites W2042138106 @default.
- W2021637685 cites W2055043387 @default.
- W2021637685 cites W2057312925 @default.
- W2021637685 cites W2061614747 @default.
- W2021637685 cites W2064190260 @default.
- W2021637685 cites W2066372429 @default.
- W2021637685 cites W2074242397 @default.
- W2021637685 cites W2094448442 @default.
- W2021637685 cites W2135361361 @default.
- W2021637685 cites W2138168728 @default.
- W2021637685 cites W2163567654 @default.
- W2021637685 cites W2226676844 @default.
- W2021637685 cites W2324841714 @default.
- W2021637685 cites W2417834997 @default.
- W2021637685 cites W2010888033 @default.
- W2021637685 cites W2130494035 @default.
- W2021637685 cites W2133636765 @default.
- W2021637685 cites W2151238743 @default.
- W2021637685 doi "https://doi.org/10.1016/s0378-1119(01)00486-3" @default.
- W2021637685 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/11418239" @default.
- W2021637685 hasPublicationYear "2001" @default.
- W2021637685 type Work @default.
- W2021637685 sameAs 2021637685 @default.
- W2021637685 citedByCount "8" @default.
- W2021637685 crossrefType "journal-article" @default.
- W2021637685 hasAuthorship W2021637685A5004126862 @default.
- W2021637685 hasAuthorship W2021637685A5036302804 @default.
- W2021637685 hasAuthorship W2021637685A5054656710 @default.
- W2021637685 hasAuthorship W2021637685A5070016214 @default.
- W2021637685 hasAuthorship W2021637685A5080838497 @default.
- W2021637685 hasAuthorship W2021637685A5089553620 @default.
- W2021637685 hasConcept C104317684 @default.
- W2021637685 hasConcept C150194340 @default.
- W2021637685 hasConcept C153911025 @default.
- W2021637685 hasConcept C162317418 @default.
- W2021637685 hasConcept C18431079 @default.
- W2021637685 hasConcept C187882448 @default.
- W2021637685 hasConcept C2779372761 @default.
- W2021637685 hasConcept C2780330800 @default.
- W2021637685 hasConcept C2781434637 @default.
- W2021637685 hasConcept C54355233 @default.
- W2021637685 hasConcept C69991583 @default.
- W2021637685 hasConcept C70721500 @default.
- W2021637685 hasConcept C86803240 @default.
- W2021637685 hasConceptScore W2021637685C104317684 @default.
- W2021637685 hasConceptScore W2021637685C150194340 @default.
- W2021637685 hasConceptScore W2021637685C153911025 @default.
- W2021637685 hasConceptScore W2021637685C162317418 @default.
- W2021637685 hasConceptScore W2021637685C18431079 @default.
- W2021637685 hasConceptScore W2021637685C187882448 @default.
- W2021637685 hasConceptScore W2021637685C2779372761 @default.
- W2021637685 hasConceptScore W2021637685C2780330800 @default.
- W2021637685 hasConceptScore W2021637685C2781434637 @default.
- W2021637685 hasConceptScore W2021637685C54355233 @default.
- W2021637685 hasConceptScore W2021637685C69991583 @default.
- W2021637685 hasConceptScore W2021637685C70721500 @default.
- W2021637685 hasConceptScore W2021637685C86803240 @default.
- W2021637685 hasIssue "2" @default.
- W2021637685 hasLocation W20216376851 @default.
- W2021637685 hasLocation W20216376852 @default.
- W2021637685 hasOpenAccess W2021637685 @default.
- W2021637685 hasPrimaryLocation W20216376851 @default.
- W2021637685 hasRelatedWork W1531528770 @default.
- W2021637685 hasRelatedWork W2021297725 @default.
- W2021637685 hasRelatedWork W2056172754 @default.
- W2021637685 hasRelatedWork W2116976163 @default.
- W2021637685 hasRelatedWork W2351824221 @default.
- W2021637685 hasRelatedWork W2378750093 @default.
- W2021637685 hasRelatedWork W2415025170 @default.
- W2021637685 hasRelatedWork W2432838036 @default.
- W2021637685 hasRelatedWork W262379139 @default.
- W2021637685 hasRelatedWork W29676990 @default.
- W2021637685 hasVolume "271" @default.
- W2021637685 isParatext "false" @default.
- W2021637685 isRetracted "false" @default.
- W2021637685 magId "2021637685" @default.
- W2021637685 workType "article" @default.