Matches in SemOpenAlex for { <https://semopenalex.org/work/W2021642010> ?p ?o ?g. }
Showing items 1 to 91 of
91
with 100 items per page.
- W2021642010 endingPage "6122" @default.
- W2021642010 startingPage "6115" @default.
- W2021642010 abstract "The maxi-K channel from bovine aortic smooth muscle consists of a pore-forming alpha subunit and a regulatory beta1 subunit that modifies the biophysical and pharmacological properties of the alpha subunit. In the present study, we examine ChTX-S10A blocking kinetics of single maxi-K channels in planar lipid bilayers from smooth muscle or from tsA-201 cells transiently transfected with either alpha or alpha+beta 1 subunits. Under low external ionic strength conditions, maxi-K channels from smooth muscle showed ChTX-S10A block times, 48 +/- 12 s, that were similar to those expressing alpha+beta 1 subunits, 51 +/- 16 s. In contrast, with the alpha subunit alone, ChTX-S10A block times were much shorter, 5 +/- 0.6 s, and were qualitatively similar to previously reported values for the skeletal muscle maxi-K channel. Increasing the external ionic strength caused a decrease in ChTX-S10A block times for maxi-K channel complexes of alpha+beta 1 subunits but not of alpha subunits alone. These findings indicate that it may be possible to predict the association of beta 1 subunits with native maxi-K channels by monitoring the kinetics of ChTX blockade of single channels, and they suggest that maxi-K channels in skeletal muscle do not contain a beta 1 subunit like the one present in smooth muscle. To further test this hypothesis, we examined the binding and cross-linking properties of [(125)I]-IbTX-D19Y/Y36F to both bovine smooth muscle and rabbit skeletal muscle membranes. [(125)I]-IbTX-D19Y/Y36F binds to rabbit skeletal muscle membranes with the same affinity as it does to smooth muscle membranes. However, specific cross-linking of [(125)I]-IbTX-D19Y/Y36F was observed into the beta 1 subunit of smooth muscle but not in skeletal muscle. Taken together, these data suggest that studies of ChTX block of single maxi-K channels provide an approach for characterizing structural and functional features of the alpha/beta 1 interaction." @default.
- W2021642010 created "2016-06-24" @default.
- W2021642010 creator A5015370358 @default.
- W2021642010 creator A5026860985 @default.
- W2021642010 creator A5066526946 @default.
- W2021642010 creator A5073329018 @default.
- W2021642010 creator A5077763814 @default.
- W2021642010 creator A5085283622 @default.
- W2021642010 date "2000-04-26" @default.
- W2021642010 modified "2023-10-18" @default.
- W2021642010 title "Interaction of Charybdotoxin S10A with Single Maxi-K Channels: Kinetics of Blockade Depend on the Presence of the β1 Subunit" @default.
- W2021642010 cites W1854945595 @default.
- W2021642010 cites W1970175010 @default.
- W2021642010 cites W2002500727 @default.
- W2021642010 cites W2014392093 @default.
- W2021642010 cites W2030669443 @default.
- W2021642010 cites W2039603477 @default.
- W2021642010 cites W2063598077 @default.
- W2021642010 cites W2131573781 @default.
- W2021642010 doi "https://doi.org/10.1021/bi992865z" @default.
- W2021642010 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/10821684" @default.
- W2021642010 hasPublicationYear "2000" @default.
- W2021642010 type Work @default.
- W2021642010 sameAs 2021642010 @default.
- W2021642010 citedByCount "15" @default.
- W2021642010 countsByYear W20216420102012 @default.
- W2021642010 countsByYear W20216420102014 @default.
- W2021642010 countsByYear W20216420102017 @default.
- W2021642010 crossrefType "journal-article" @default.
- W2021642010 hasAuthorship W2021642010A5015370358 @default.
- W2021642010 hasAuthorship W2021642010A5026860985 @default.
- W2021642010 hasAuthorship W2021642010A5066526946 @default.
- W2021642010 hasAuthorship W2021642010A5073329018 @default.
- W2021642010 hasAuthorship W2021642010A5077763814 @default.
- W2021642010 hasAuthorship W2021642010A5085283622 @default.
- W2021642010 hasConcept C104292427 @default.
- W2021642010 hasConcept C104317684 @default.
- W2021642010 hasConcept C105702510 @default.
- W2021642010 hasConcept C121332964 @default.
- W2021642010 hasConcept C12554922 @default.
- W2021642010 hasConcept C148898269 @default.
- W2021642010 hasConcept C185592680 @default.
- W2021642010 hasConcept C199360897 @default.
- W2021642010 hasConcept C2776174256 @default.
- W2021642010 hasConcept C2779959927 @default.
- W2021642010 hasConcept C2780419564 @default.
- W2021642010 hasConcept C41008148 @default.
- W2021642010 hasConcept C55493867 @default.
- W2021642010 hasConcept C62520636 @default.
- W2021642010 hasConcept C83730767 @default.
- W2021642010 hasConcept C83743174 @default.
- W2021642010 hasConcept C86803240 @default.
- W2021642010 hasConceptScore W2021642010C104292427 @default.
- W2021642010 hasConceptScore W2021642010C104317684 @default.
- W2021642010 hasConceptScore W2021642010C105702510 @default.
- W2021642010 hasConceptScore W2021642010C121332964 @default.
- W2021642010 hasConceptScore W2021642010C12554922 @default.
- W2021642010 hasConceptScore W2021642010C148898269 @default.
- W2021642010 hasConceptScore W2021642010C185592680 @default.
- W2021642010 hasConceptScore W2021642010C199360897 @default.
- W2021642010 hasConceptScore W2021642010C2776174256 @default.
- W2021642010 hasConceptScore W2021642010C2779959927 @default.
- W2021642010 hasConceptScore W2021642010C2780419564 @default.
- W2021642010 hasConceptScore W2021642010C41008148 @default.
- W2021642010 hasConceptScore W2021642010C55493867 @default.
- W2021642010 hasConceptScore W2021642010C62520636 @default.
- W2021642010 hasConceptScore W2021642010C83730767 @default.
- W2021642010 hasConceptScore W2021642010C83743174 @default.
- W2021642010 hasConceptScore W2021642010C86803240 @default.
- W2021642010 hasIssue "20" @default.
- W2021642010 hasLocation W20216420101 @default.
- W2021642010 hasLocation W20216420102 @default.
- W2021642010 hasOpenAccess W2021642010 @default.
- W2021642010 hasPrimaryLocation W20216420101 @default.
- W2021642010 hasRelatedWork W1487293552 @default.
- W2021642010 hasRelatedWork W1495434193 @default.
- W2021642010 hasRelatedWork W1993563590 @default.
- W2021642010 hasRelatedWork W2002291752 @default.
- W2021642010 hasRelatedWork W2081370552 @default.
- W2021642010 hasRelatedWork W2082438131 @default.
- W2021642010 hasRelatedWork W2105517484 @default.
- W2021642010 hasRelatedWork W2115892452 @default.
- W2021642010 hasRelatedWork W815604900 @default.
- W2021642010 hasRelatedWork W2407877338 @default.
- W2021642010 hasVolume "39" @default.
- W2021642010 isParatext "false" @default.
- W2021642010 isRetracted "false" @default.
- W2021642010 magId "2021642010" @default.
- W2021642010 workType "article" @default.