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• W2021647512 abstract "Event Abstract Back to Event Differential effects of the secretome of mesenchymal progenitors from the umbilical cor on the survival and viability of hippocampal, cerebellar and cortical primary cultures of neurons. J. S. Fraga1*, N. A. Silva1, N. M. Neves1, R. L. Reis1, Nuno Sousa1 and A. J. Salgado1 1 University of Minho, Life and Health Sciences Institute (ICVS), School of Health Sciences, Portugal Recent studies have shown that mesenchymal like adult stem cells isolated from umbilical cord’s Wharton Jelly might be used as possible therapeutical agents to treat disorders associated to the central nervous system (CNS). It has been hypothesized that their secretome may play a role on these effects. We have previously shown that these cells produce neuroregulatory factors that modulate the survival of hippocampal neurons. However it is expected that different populations of neurons react differently to the same secretome. Therefore the objective of the present work was to understand the effects of the secretome of a population of mesenchymal progenitor from the umbilical cord’s Wharton Jelly, known as Human Umbilical Cord Perivascular Cells (HUCPVCs), on post-natal neurons isolated from different areas in the brain, namely hippocampus, cerebellum and cortex.HUCPVCs were isolated from the primitive connective tissue present in umbilical cord and further cultured for 3 days at 4000cells/cm2. On day 3 the medium was replaced and 24h, 48h, 72h and 96h thereafter HUCPVCs conditioned media (CM) was collected. The diverse neuronal populations (cortical, cerebellar and hippocampal) were isolated from P4 newborn wistar rats. Upon isolation they were exposed to HUCPVCs CM for 7 days. Control cultures were incubated with standard Neurobasal A medium. Cell viability was assayed by the MTS test while cell proliferation was assessed by a 24h BrdU incubation from days 6 to 7. Cell counts were performed on immunostained cultures for MAP-2 (neurons) and GFAP (astrocytes).For hippocampal neurons CM 96h lead to an increase of the metabolic viability and cell proliferation. Similar effects were also found for cortical neurons. In this particular case CM 96h incubation lead to a six folds increase when compared to controls. Important to mention is that in control cultures no cortical neurons could be observed, while with CM 24h to 72h incubated cultures the total percentage was around 40% of cortical neurons. Concerning cerebellar cultures the highest value of metabolic viability and MAP-2 positive cells was observed with CM 24h whereas CM 48h induced higher levels of cell proliferation. As a conclusion it can be stated that there is a clear evidence that the secretome of HUCPVCs CM leads to different reactions on post- natal neurons from different sources. Conference: 11th Meeting of the Portuguese Society for Neuroscience, Braga, Portugal, 4 Jun - 6 Jun, 2009. Presentation Type: Poster Presentation Topic: Abstracts Citation: Fraga JS, Silva NA, Neves NM, Reis RL, Sousa N and Salgado AJ (2009). Differential effects of the secretome of mesenchymal progenitors from the umbilical cor on the survival and viability of hippocampal, cerebellar and cortical primary cultures of neurons.. Front. Neurosci. Conference Abstract: 11th Meeting of the Portuguese Society for Neuroscience. doi: 10.3389/conf.neuro.01.2009.11.086 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 10 Aug 2009; Published Online: 10 Aug 2009. * Correspondence: J. S Fraga, University of Minho, Life and Health Sciences Institute (ICVS), School of Health Sciences, Minho, Portugal, joanafraga@ecsaude.uminho.pt Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers J. S Fraga N. A Silva N. M Neves R. L Reis Nuno Sousa A. J Salgado Google J. S Fraga N. A Silva N. M Neves R. L Reis Nuno Sousa A. J Salgado Google Scholar J. S Fraga N. A Silva N. M Neves R. L Reis Nuno Sousa A. J Salgado PubMed J. S Fraga N. A Silva N. M Neves R. L Reis Nuno Sousa A. J Salgado Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page." @default.
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• W2021647512 date "2009-01-01" @default.
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• W2021647512 title "Differential effects of the secretome of mesenchymal progenitors from the umbilical cor on the survival and viability of hippocampal, cerebellar and cortical primary cultures of neurons." @default.
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