FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2021651344> ?p ?o ?g. }

• W2021651344 endingPage "130" @default.
• W2021651344 startingPage "123" @default.
• W2021651344 abstract "Bacterial peptidyl-tRNA hydrolase (Pth) activity ensures the rapid recycling of peptidyl-tRNAs that result from premature termination of translation. Pth has been shown to be essential for growth in Escherichia coli suggesting that its homologue in Staphylococcus aureus is a potential molecular therapeutic target for the development of antibacterial agents. In this report we describe the cloning of a DNA fragment (573 bp) containing the pth gene from a S. aureus (strain ISP3) genomic DNA library. Analysis of the predicted polypeptide sequence from the pth gene showed that the protein shared complete conservation of the three residues thought to be involved in the active site of E. coli Pth. The gene was cloned into a pQE-60 expression vector and expressed in E. coli, and the resulting His-tagged Pth protein was purified to greater than 95% purity from the soluble portion of the E. coli lysate in a single chromatographic step. His-tagged Pth was shown to be biologically active by its ability to hydrolyze diacetyl-[(3)H]Lys-tRNA(Lys) in a time- and concentration-dependent manner. Optimum hydrolyzing activity of Pth occurred at a pH value of 7.0 and a MgCl(2) concentration of 5 mM. The K(m) of the diacetyl-[(3)H]-Lys-tRNA(Lys) substrate for S. aureus Pth was determined to be 2.8 microM. A far UV circular dichroism spectrum revealed that His-tagged S. aureus Pth appears to have a structured core predominated by beta-sheet." @default.
• W2021651344 created "2016-06-24" @default.
• W2021651344 creator A5016572767 @default.
• W2021651344 creator A5019503017 @default.
• W2021651344 creator A5026338910 @default.
• W2021651344 creator A5039727195 @default.
• W2021651344 creator A5067326334 @default.
• W2021651344 creator A5073181339 @default.
• W2021651344 creator A5076956431 @default.
• W2021651344 creator A5082948668 @default.
• W2021651344 creator A5088542908 @default.
• W2021651344 date "2002-02-01" @default.
• W2021651344 modified "2023-10-17" @default.
• W2021651344 title "Expression, Purification, and Characterization of Peptidyl-tRNA Hydrolase from Staphylococcus aureus" @default.
• W2021651344 cites W103355764 @default.
• W2021651344 cites W1480942814 @default.
• W2021651344 cites W1510119324 @default.
• W2021651344 cites W1518434992 @default.
• W2021651344 cites W1619272169 @default.
• W2021651344 cites W1670888403 @default.
• W2021651344 cites W1969019274 @default.
• W2021651344 cites W1977181618 @default.
• W2021651344 cites W1977393741 @default.
• W2021651344 cites W1977474319 @default.
• W2021651344 cites W1981776605 @default.
• W2021651344 cites W1982013617 @default.
• W2021651344 cites W2016349668 @default.
• W2021651344 cites W2053058161 @default.
• W2021651344 cites W2068325009 @default.
• W2021651344 cites W2071758457 @default.
• W2021651344 cites W2084586003 @default.
• W2021651344 cites W2086762743 @default.
• W2021651344 cites W2093986976 @default.
• W2021651344 cites W2097754218 @default.
• W2021651344 cites W2148382456 @default.
• W2021651344 cites W2170367123 @default.
• W2021651344 cites W2329458958 @default.
• W2021651344 doi "https://doi.org/10.1006/prep.2001.1540" @default.
• W2021651344 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/11812233" @default.
• W2021651344 hasPublicationYear "2002" @default.
• W2021651344 type Work @default.
• W2021651344 sameAs 2021651344 @default.
• W2021651344 citedByCount "10" @default.
• W2021651344 countsByYear W20216513442012 @default.
• W2021651344 countsByYear W20216513442016 @default.
• W2021651344 countsByYear W20216513442018 @default.
• W2021651344 countsByYear W20216513442021 @default.
• W2021651344 crossrefType "journal-article" @default.
• W2021651344 hasAuthorship W2021651344A5016572767 @default.
• W2021651344 hasAuthorship W2021651344A5019503017 @default.
• W2021651344 hasAuthorship W2021651344A5026338910 @default.
• W2021651344 hasAuthorship W2021651344A5039727195 @default.
• W2021651344 hasAuthorship W2021651344A5067326334 @default.
• W2021651344 hasAuthorship W2021651344A5073181339 @default.
• W2021651344 hasAuthorship W2021651344A5076956431 @default.
• W2021651344 hasAuthorship W2021651344A5082948668 @default.
• W2021651344 hasAuthorship W2021651344A5088542908 @default.
• W2021651344 hasConcept C104317684 @default.
• W2021651344 hasConcept C153911025 @default.
• W2021651344 hasConcept C181199279 @default.
• W2021651344 hasConcept C185592680 @default.
• W2021651344 hasConcept C2779489039 @default.
• W2021651344 hasConcept C2780120296 @default.
• W2021651344 hasConcept C40767141 @default.
• W2021651344 hasConcept C523546767 @default.
• W2021651344 hasConcept C54355233 @default.
• W2021651344 hasConcept C547475151 @default.
• W2021651344 hasConcept C55493867 @default.
• W2021651344 hasConcept C86803240 @default.
• W2021651344 hasConceptScore W2021651344C104317684 @default.
• W2021651344 hasConceptScore W2021651344C153911025 @default.
• W2021651344 hasConceptScore W2021651344C181199279 @default.
• W2021651344 hasConceptScore W2021651344C185592680 @default.
• W2021651344 hasConceptScore W2021651344C2779489039 @default.
• W2021651344 hasConceptScore W2021651344C2780120296 @default.
• W2021651344 hasConceptScore W2021651344C40767141 @default.
• W2021651344 hasConceptScore W2021651344C523546767 @default.
• W2021651344 hasConceptScore W2021651344C54355233 @default.
• W2021651344 hasConceptScore W2021651344C547475151 @default.
• W2021651344 hasConceptScore W2021651344C55493867 @default.
• W2021651344 hasConceptScore W2021651344C86803240 @default.
• W2021651344 hasIssue "1" @default.
• W2021651344 hasLocation W20216513441 @default.
• W2021651344 hasLocation W20216513442 @default.
• W2021651344 hasOpenAccess W2021651344 @default.
• W2021651344 hasPrimaryLocation W20216513441 @default.
• W2021651344 hasRelatedWork W1520209912 @default.
• W2021651344 hasRelatedWork W2050917233 @default.
• W2021651344 hasRelatedWork W2146613452 @default.
• W2021651344 hasRelatedWork W2274607931 @default.
• W2021651344 hasRelatedWork W2360987025 @default.
• W2021651344 hasRelatedWork W2368867676 @default.
• W2021651344 hasRelatedWork W2387178301 @default.
• W2021651344 hasRelatedWork W2992955585 @default.
• W2021651344 hasRelatedWork W3046976449 @default.
• W2021651344 hasRelatedWork W3180967004 @default.
• W2021651344 hasVolume "24" @default.
• W2021651344 isParatext "false" @default.

• next