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• W2021657259 abstract "Strain Q of cucumber mosiac virus (QCMV) from Queensland, Australia, contains 18% ribonucleic acid (RNA) which has a base ratio (moles %) of adenine 22.4%, guanine 24.7%, cytosine 22.8%, and uracil 30.1%. The molecular weight of the virus is approximately 5.8 × 106, based on a sedimentation coefficient of 98.6 S and a diffusion coefficient of 1.20 × 107 cm2 per second. Hence the virus contains about 1.0 × 106 molecular weight units of RNA. The electrophoretic mobility of QCMV is −7.2 × 10−5 cm2 per volt per second at pH 9 and −4.0 × 10−5 cm2 per volt per second at pH 7.4. Electron micrographs of QCMV preparations show particles about 28 mμ in diameter when stained in phosphotungstic acid (PTA) or uranyl acetate and about 42 mμ when shadowed with gold-palladium. Partially purified QCMV in 0.005 M borate buffer, pH 9, remained infectious for several months when stored at 0–4°C. Such preparations retained some infectivity after heating between 70 and 80° for 10 minutes whereas freshly extracted sap from infected cucumber plants was completely inactivated after 10 minutes at 60°. QCMV precipitated in the pH range 4.3–5.5. At 0°C, QCMV was unaffected by the presence of NaCl at concentrations up to about 0.25 M, but was rapidly precipitated on mild heating. QCMV is unstable and slowly disrupts in 2% PTA, pH 7. It is also degraded in 1 M CaCl2 and 2 M LiCl and the RNA precipitates quantitatively leaving most of the protein in solution. CaCl2-precipitated QCMV-RNA could not be dissolved in neutral buffers, but the RNA precipitated with LiCl was readily resuspended and shown to be several times more infectious than phenol-extracted RNA. QCMV has been shown to be antigenically similar to the Y strain of CMV (CMV-Y) from North America. However, these two strains appear to differ in some respects which are discussed." @default.
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• W2021657259 date "1966-04-01" @default.
• W2021657259 modified "2023-10-16" @default.
• W2021657259 title "Some properties of purified cucumber mosaic virus (Q strain)" @default.
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• W2021657259 doi "https://doi.org/10.1016/0042-6822(66)90257-1" @default.
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