Matches in SemOpenAlex for { <https://semopenalex.org/work/W2021669901> ?p ?o ?g. }
Showing items 1 to 90 of
90
with 100 items per page.
- W2021669901 endingPage "1477" @default.
- W2021669901 startingPage "1475" @default.
- W2021669901 abstract "Capillary electrophoresis (CE) for the simultaneous and precise quantification of human insulin (hI), proinsulin (hPI) and intermediate forms (des 31, 32; split 65-66 and des 64, 65), released in culture media by engineered cells, is described. Analytical conditions for standard proteins were optimized using a bare silica capillary (20 cm X 50 microm internal diameter). Proteins were monitored at 200 nm and separated at constant voltage. Culture supernatants (12-24 mL) were purified on Sep-Pak Vac C18 cartridges, recovered in 1 mL of acetonitrile:trifluoracetic acid mixture (60:40, v:v), concentrated, ultrafiltered and injected into CE. Protein recovery was 85+/-14% (n = 5, mean+/-standard deviation) with a sensitivity limit of 0.5 nmol/L in the culture media, corresponding to 2 fmol injected in 22 nL. Using the CE method, it was possible to detect and quantify, with precision and accuracy, the release of hPI, hI and intermediate forms directly in the cell culture media, and to compare the proteic pattern released from engineered cells transduced with different hPI gene constructs." @default.
- W2021669901 created "2016-06-24" @default.
- W2021669901 creator A5010653333 @default.
- W2021669901 creator A5015698650 @default.
- W2021669901 creator A5030216074 @default.
- W2021669901 creator A5054856956 @default.
- W2021669901 creator A5080534919 @default.
- W2021669901 date "1998-06-01" @default.
- W2021669901 modified "2023-09-26" @default.
- W2021669901 title "Capillary electrophoresis for simultaneous quantification of human proinsulin, insulin and intermediate forms" @default.
- W2021669901 cites W1487163547 @default.
- W2021669901 cites W1942616381 @default.
- W2021669901 cites W1990362866 @default.
- W2021669901 cites W1997507020 @default.
- W2021669901 cites W2020821717 @default.
- W2021669901 cites W2033730384 @default.
- W2021669901 cites W2045773412 @default.
- W2021669901 cites W2047340098 @default.
- W2021669901 cites W2069264186 @default.
- W2021669901 cites W2094913421 @default.
- W2021669901 cites W2123193380 @default.
- W2021669901 cites W2124706352 @default.
- W2021669901 cites W2124765872 @default.
- W2021669901 cites W2148576568 @default.
- W2021669901 cites W2150987825 @default.
- W2021669901 cites W4247281676 @default.
- W2021669901 doi "https://doi.org/10.1002/elps.1150190843" @default.
- W2021669901 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/9694298" @default.
- W2021669901 hasPublicationYear "1998" @default.
- W2021669901 type Work @default.
- W2021669901 sameAs 2021669901 @default.
- W2021669901 citedByCount "13" @default.
- W2021669901 countsByYear W20216699012012 @default.
- W2021669901 countsByYear W20216699012013 @default.
- W2021669901 countsByYear W20216699012014 @default.
- W2021669901 countsByYear W20216699012015 @default.
- W2021669901 countsByYear W20216699012016 @default.
- W2021669901 countsByYear W20216699012022 @default.
- W2021669901 crossrefType "journal-article" @default.
- W2021669901 hasAuthorship W2021669901A5010653333 @default.
- W2021669901 hasAuthorship W2021669901A5015698650 @default.
- W2021669901 hasAuthorship W2021669901A5030216074 @default.
- W2021669901 hasAuthorship W2021669901A5054856956 @default.
- W2021669901 hasAuthorship W2021669901A5080534919 @default.
- W2021669901 hasConcept C119128265 @default.
- W2021669901 hasConcept C134018914 @default.
- W2021669901 hasConcept C159985019 @default.
- W2021669901 hasConcept C185592680 @default.
- W2021669901 hasConcept C192562407 @default.
- W2021669901 hasConcept C196806460 @default.
- W2021669901 hasConcept C2779306644 @default.
- W2021669901 hasConcept C2900643 @default.
- W2021669901 hasConcept C40250595 @default.
- W2021669901 hasConcept C43617362 @default.
- W2021669901 hasConcept C86803240 @default.
- W2021669901 hasConcept C90061646 @default.
- W2021669901 hasConceptScore W2021669901C119128265 @default.
- W2021669901 hasConceptScore W2021669901C134018914 @default.
- W2021669901 hasConceptScore W2021669901C159985019 @default.
- W2021669901 hasConceptScore W2021669901C185592680 @default.
- W2021669901 hasConceptScore W2021669901C192562407 @default.
- W2021669901 hasConceptScore W2021669901C196806460 @default.
- W2021669901 hasConceptScore W2021669901C2779306644 @default.
- W2021669901 hasConceptScore W2021669901C2900643 @default.
- W2021669901 hasConceptScore W2021669901C40250595 @default.
- W2021669901 hasConceptScore W2021669901C43617362 @default.
- W2021669901 hasConceptScore W2021669901C86803240 @default.
- W2021669901 hasConceptScore W2021669901C90061646 @default.
- W2021669901 hasIssue "8-9" @default.
- W2021669901 hasLocation W20216699011 @default.
- W2021669901 hasLocation W20216699012 @default.
- W2021669901 hasOpenAccess W2021669901 @default.
- W2021669901 hasPrimaryLocation W20216699011 @default.
- W2021669901 hasRelatedWork W1966225535 @default.
- W2021669901 hasRelatedWork W2018283550 @default.
- W2021669901 hasRelatedWork W2022433216 @default.
- W2021669901 hasRelatedWork W2044824815 @default.
- W2021669901 hasRelatedWork W2077855486 @default.
- W2021669901 hasRelatedWork W2086792757 @default.
- W2021669901 hasRelatedWork W2093836191 @default.
- W2021669901 hasRelatedWork W2469372477 @default.
- W2021669901 hasRelatedWork W4242604825 @default.
- W2021669901 hasRelatedWork W882847974 @default.
- W2021669901 hasVolume "19" @default.
- W2021669901 isParatext "false" @default.
- W2021669901 isRetracted "false" @default.
- W2021669901 magId "2021669901" @default.
- W2021669901 workType "article" @default.