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- W2021678204 abstract "A new highly sensitive gas chromatographic method for the separate measurement of microsomal oxide synthetase (aniline, reduced-flavoprotein: oxygen oxidoreductase (4-hydroxylating), EC 1.14.14.1) and oxide hydratase (glycol hydro-lyase (epoxide-forming), EC 4.21.63) activities by using styrene or styrene oxide as substrate is presented. The assay has been developed in order to permit the evaluation of the true kinetic parameters of the two enzymes, respectively, catalysing the activation and desactivation processes of styrene. In the presence of a microsomal suspension and a NADPH generating system, styrene is converted, under the influence of oxide synthetase, into styrene oxide, a certain amount of which is hydrated into styrene glycol, either spontaneously or enzymatically by the microsomal oxide hydratase; the remaining fraction of the oxide is chemically hydrated in acidic conditions. The total amount of styrene glycol formed is quantitated by electron-capture gas chromatography after its conversion into pentafluobenzoyl derivative. For the measurement of oxide hydratase activity, styrene glycol formed after incubation of styrene oxide with the microsomal fraction is estimated as before. The kinetic data obtained in this study demonstrate that, at low protein concentrations, the affinity of oxide synthetase for styrene is about 20 times as low as that of the oxide hydratase for styrene oxide." @default.
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- W2021678204 date "1978-09-01" @default.
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- W2021678204 title "Determination of oxide synthetase and hydratase activities by a new highly sensitive gas chromatographic method using styrene and styrene oxide as substrates" @default.
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- W2021678204 doi "https://doi.org/10.1016/0005-2744(78)90291-7" @default.
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