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• W2021721922 abstract "It has been demonstrated that immuno-modu-lating mechanisms induced by epimastigotes ofTrypanosoma rangeli, an assumed harmless hu-man parasite, may have relevance to protectionagainst Trypanosoma cruzi, the causative organ-ism of Chagas’ disease (B Basso et al. 1991 Am JTrop Med Hyg 44: 413-419). Evidently, this find-ing is related to the previously demonstrated an-tigenic similarity, between these parasites (DAfchain et al. 1979 J Parasitol 65: 507-514, BBasso et al. 1989 Rev Lat-amer Microbiol 31 : 141-146, M Grogl, RE Kuhn 1984 J Parasitol 70 : 822-824, F Guhl, CJ Marinkelle 1982 Ana Trop MedParasitol 76: 361). However, at the present timeit is not known which T. rangeli antigens can elicitantibodies capable to recognize T. cruzi compo-nents. In order to clarify this matter, we used theimmunoblotting technique to delineate the cross-reactivity among the immunogenic components ofthese parasites.The parasites used were isolated from humansin central Panama. Clones derived from single cellisolates (JA Dvorak 1985, Rev Soc Bras Med Trop18 (Suppl): 29-38) of T. rangeli (LMCL2) and T.cruzi (MA-081A) were characterized by studiesof morphology, intracellular multiplication mea-sured in vitro and infectivity for vector salivaryglands. Flagellates were cultivated and lyophilizedfollowing procedures designed previously (NHVattuone, JF Yanovsky 1971 Exp Parasitol 30:349-355, A Saldana 1990 ImmunoparasitologicalStudies of Trypanosoma cruzi clones from Panama ,MSc Thesis, I. Karolinska-Stockholm, 90 pp). Thelyophilized T. rangeli and T. cruzi epimastigoteswere used in the production of mouse antibodiesas ascitic fluid (AS Tung et al. 1976 J Immunol116: 676-681, AE Horna 1992 Biochemical andImmunological Characterization of Trypanosomarangeli (Tejera 1920) strains affecting rural popu-lations of Central and South America. MSc The-sis, I. Karolinska-Stockholm, 100 pp). To demon-strate the antibody cross-reactivity we followedprocedures described earlier (EC Rostjord et al.1990 J Parasitol 76: 698-702).Previous reports suggested that exposure to T.rangeli antigens might modify the pathology dueto T. cruzi (Grogl, Kuhn loc. cit., F Guhl et al.1987 Parasitol 94: 475-484, L Hudson et al. 1988Parasitol 96: 449-460). Nevertheless, as far as weknow , the first work that demonstrated a partialresistance in T. rangeli-immunized mice againstT. cruzi infection was done by Basso et al. (loc.cit.). However, the studies of T. rangeli immuno-genic components seems to be just beginning.Our results (Figs 1, 2) revealed that there areseveral common epitopes among T. rangeli anti-gens and between T. rangeli and T. cruzi polypep-tides. The antibodies eluted from the regions A,B, C, D, E and F recognized additional bands tothe bands from which they were separated eitherin T. rangeli or T. cruzi immunoblotting profiles.Most of them cross-reacted with antigens in re-gion B ( 81, 76 and 71 Kda). However, when theantibodies eluted from regions of 34 (G), 29 (H)and 24 Kda (I) were used, more specificity wasfound. The antibodies from the bands of 34 and29 Kda recognized, without differences, antigenswith similar molecular weights in the T. rangelior T. cruzi profiles. This may, therefore, representthe expression of two antigens, highly conserved,which apparently are a second group ofimmunodominant determinants responsible for theobserved cross-reactivity.An additional finding was the specific recog-nition pattern noted with the antibodies from the24 Kda region, apparently this antigen shows athird restrictive group of immunodominantepitopes, expressed either in the 24 Kda polypep-tide of T. rangeli and in the 23 Kda region of T.cruzi.Even with these cross-reactions, it should bekept in mind, that it is possible to find specificepitopes in each one of these molecules. The iden-tification of specific epitopes recognized by mono-clonal antibodies should facilitate studies whichaim at settling this possibility.Finally, research on purification and immu-nochemistry of these T. rangeli antigens, whichcross-reacted with T. cruzi components, could beimportant on the diagnostic and management ofChagas’ disease." @default.
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• W2021721922 title "Trypanosoma rangeli and Trypanosoma cruzi: cross-reaction among their immunogenic components" @default.
• W2021721922 doi "https://doi.org/10.1590/s0074-02761996000100013" @default.
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