FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2021734722> ?p ?o ?g. }

• W2021734722 endingPage "6268" @default.
• W2021734722 startingPage "6262" @default.
• W2021734722 abstract "A cDNA encoding the viral protease from the 3C region of human rhinovirus type 14 was expressed in Escherichia coli through the use of a periplasmic secretion vector. The recombinant protease contained an eight amino acid N-terminal extension that enabled its detection by a specific antibody. It was expressed at a level of approximately 1 mg/L of E. coli culture. Biological activity of the protease was assessed in vitro by using a chemically synthesized peptide consisting of a consensus picornavirus protease cleavage site, Arg-Ala-Glu-Leu-Gln-Gly-Pro-Tyr-Asp-Glu. The peptide was cleaved by the recombinant protease at the Gln-Gly bond, generating the product peptides Arg-Ala-Glu-Leu-Gln and Gly-Pro-Tyr-Asp-Glu, which could be separated from the substrate peptide by reversed-phase HPLC. An in vitro assay for the rhinovirus 3C protease was developed by observing the rate of disappearance of the substrate peak from chromatograms of the supernatants of digestion mixtures." @default.
• W2021734722 created "2016-06-24" @default.
• W2021734722 creator A5011420454 @default.
• W2021734722 creator A5035349905 @default.
• W2021734722 creator A5041060382 @default.
• W2021734722 creator A5046955485 @default.
• W2021734722 creator A5072612404 @default.
• W2021734722 creator A5079781502 @default.
• W2021734722 date "1988-08-23" @default.
• W2021734722 modified "2023-09-23" @default.
• W2021734722 title "Human rhinovirus 3C protease: cloning and expression of an active form in Escherichia coli" @default.
• W2021734722 cites W1535133733 @default.
• W2021734722 cites W1981163610 @default.
• W2021734722 cites W1995584639 @default.
• W2021734722 cites W2004737963 @default.
• W2021734722 cites W2024889628 @default.
• W2021734722 cites W2026066831 @default.
• W2021734722 cites W2037263249 @default.
• W2021734722 cites W2051468219 @default.
• W2021734722 cites W2058423707 @default.
• W2021734722 cites W2060932164 @default.
• W2021734722 cites W2071477986 @default.
• W2021734722 cites W2079487259 @default.
• W2021734722 cites W2081231862 @default.
• W2021734722 cites W2081910830 @default.
• W2021734722 cites W2082228480 @default.
• W2021734722 cites W2086359368 @default.
• W2021734722 cites W2086937185 @default.
• W2021734722 cites W2089617243 @default.
• W2021734722 cites W2091239909 @default.
• W2021734722 cites W2100837269 @default.
• W2021734722 cites W2101108802 @default.
• W2021734722 cites W2109039986 @default.
• W2021734722 cites W2138270253 @default.
• W2021734722 cites W2154632747 @default.
• W2021734722 cites W4251918261 @default.
• W2021734722 doi "https://doi.org/10.1021/bi00417a010" @default.
• W2021734722 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/2851319" @default.
• W2021734722 hasPublicationYear "1988" @default.
• W2021734722 type Work @default.
• W2021734722 sameAs 2021734722 @default.
• W2021734722 citedByCount "37" @default.
• W2021734722 countsByYear W20217347222012 @default.
• W2021734722 countsByYear W20217347222013 @default.
• W2021734722 countsByYear W20217347222020 @default.
• W2021734722 countsByYear W20217347222022 @default.
• W2021734722 crossrefType "journal-article" @default.
• W2021734722 hasAuthorship W2021734722A5011420454 @default.
• W2021734722 hasAuthorship W2021734722A5035349905 @default.
• W2021734722 hasAuthorship W2021734722A5041060382 @default.
• W2021734722 hasAuthorship W2021734722A5046955485 @default.
• W2021734722 hasAuthorship W2021734722A5072612404 @default.
• W2021734722 hasAuthorship W2021734722A5079781502 @default.
• W2021734722 hasConcept C104317684 @default.
• W2021734722 hasConcept C109110057 @default.
• W2021734722 hasConcept C153911025 @default.
• W2021734722 hasConcept C159047783 @default.
• W2021734722 hasConcept C167625842 @default.
• W2021734722 hasConcept C181199279 @default.
• W2021734722 hasConcept C185592680 @default.
• W2021734722 hasConcept C187882448 @default.
• W2021734722 hasConcept C2522874641 @default.
• W2021734722 hasConcept C2776625791 @default.
• W2021734722 hasConcept C2776714187 @default.
• W2021734722 hasConcept C2777833472 @default.
• W2021734722 hasConcept C2779278377 @default.
• W2021734722 hasConcept C2779281246 @default.
• W2021734722 hasConcept C40767141 @default.
• W2021734722 hasConcept C547475151 @default.
• W2021734722 hasConcept C55493867 @default.
• W2021734722 hasConcept C86803240 @default.
• W2021734722 hasConceptScore W2021734722C104317684 @default.
• W2021734722 hasConceptScore W2021734722C109110057 @default.
• W2021734722 hasConceptScore W2021734722C153911025 @default.
• W2021734722 hasConceptScore W2021734722C159047783 @default.
• W2021734722 hasConceptScore W2021734722C167625842 @default.
• W2021734722 hasConceptScore W2021734722C181199279 @default.
• W2021734722 hasConceptScore W2021734722C185592680 @default.
• W2021734722 hasConceptScore W2021734722C187882448 @default.
• W2021734722 hasConceptScore W2021734722C2522874641 @default.
• W2021734722 hasConceptScore W2021734722C2776625791 @default.
• W2021734722 hasConceptScore W2021734722C2776714187 @default.
• W2021734722 hasConceptScore W2021734722C2777833472 @default.
• W2021734722 hasConceptScore W2021734722C2779278377 @default.
• W2021734722 hasConceptScore W2021734722C2779281246 @default.
• W2021734722 hasConceptScore W2021734722C40767141 @default.
• W2021734722 hasConceptScore W2021734722C547475151 @default.
• W2021734722 hasConceptScore W2021734722C55493867 @default.
• W2021734722 hasConceptScore W2021734722C86803240 @default.
• W2021734722 hasIssue "17" @default.
• W2021734722 hasLocation W20217347221 @default.
• W2021734722 hasLocation W20217347222 @default.
• W2021734722 hasOpenAccess W2021734722 @default.
• W2021734722 hasPrimaryLocation W20217347221 @default.
• W2021734722 hasRelatedWork W2001777806 @default.
• W2021734722 hasRelatedWork W2338137066 @default.
• W2021734722 hasRelatedWork W2350146650 @default.
• W2021734722 hasRelatedWork W2363249581 @default.

• next