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- W2021768082 abstract "A high-performance liquid chromatographic procedure for the determination of cocaine and selected metabolites (benzoylecgonine, norcocaine and benzoylnorecgonine) from human and canine serum has been developed. The analytes are extracted from 0.5-ml serum samples using strong cation-exchange and octadecylsilane solid-phase extraction columns. Chromatographic separation was accomplished on coupled cyanopropyl and silica columns using acetonitrile-6.25 mM phosphate buffer, pH 2.9 (80:20 v/v) as eluent and a flow-rate of 1.1 ml/min. Low-wavelength UV detection (228 nm, cadmium lamp) allowed detection of 1 ng/ml for each analyte. The assay was linear from 0 to 200 ng/ml (r2 = 0.997–0.999, n = 15 for each analyte) and showed good precision (relative standard deviation = 0.66–13.8%, n = 6) and accuracy (98–115% relative recovery)." @default.
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- W2021768082 title "Determination of cocaine and selected metabolites in canine and human serum by reversed-phase high-performance liquid chromatography on coupled cyanopropyl and silica columns" @default.
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- W2021768082 doi "https://doi.org/10.1016/s0378-4347(00)82618-3" @default.
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