FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2021777797> ?p ?o ?g. }

• W2021777797 endingPage "842" @default.
• W2021777797 startingPage "837" @default.
• W2021777797 abstract "The soluble human interleukin-6 receptor (shIL6R) was purified from human plasma. In a single immunoaffinity purification step a 140000-fold enrichment with a yield of 95% was achieved. A subsequent IL-6 affinity chromatography resulted in a homogeneous receptor preparation but only in a yield of less than 5%. The biological activity of the soluble receptor was clearly demonstrated by its ability to induce the synthesis of the acute-phase protein 1-antichymotrypsin in HepG2 cells stably transfected with IL-6. Upon gel filtration, the native shIL6R showed an apparent molecular mass of 93 kDa. Analysis by SDS/PAGE revealed an apparent molecular mass of 65 kDa for the soluble receptor. Deglycosylation with peptide N-glycosidase F led to a shift in molecular mass from 65 kDa to 45 kDa. It has previously been shown that the shIL6R can be generated by shedding the membrane-bound form or by expression of an alternatively spliced mRNA. Here we show that the shIL6R isolated from human plasma is recognized by an affinity-purified peptide antibody raised against an amino acid sequence unique for the alternatively spliced isoform. Thus, the shIL6R isoform generated through alternative splicing which has been previously detected in supernatants of cultured cell lines is also an in vivo product circulating in human plasma." @default.
• W2021777797 created "2016-06-24" @default.
• W2021777797 creator A5016159915 @default.
• W2021777797 creator A5025374231 @default.
• W2021777797 creator A5027069639 @default.
• W2021777797 creator A5028612887 @default.
• W2021777797 creator A5036852859 @default.
• W2021777797 creator A5038296391 @default.
• W2021777797 creator A5054786640 @default.
• W2021777797 creator A5075814041 @default.
• W2021777797 creator A5079991502 @default.
• W2021777797 date "1996-03-15" @default.
• W2021777797 modified "2023-10-16" @default.
• W2021777797 title "Purification and Characterization of the Soluble Interleukin-6 Receptor from Human Plasma and Identification of An Isoform Generated through Alternative Splicing" @default.
• W2021777797 cites W1493873711 @default.
• W2021777797 cites W1575122167 @default.
• W2021777797 cites W1596383188 @default.
• W2021777797 cites W1597952938 @default.
• W2021777797 cites W1671275184 @default.
• W2021777797 cites W1776476655 @default.
• W2021777797 cites W1777081626 @default.
• W2021777797 cites W1796351973 @default.
• W2021777797 cites W1968814677 @default.
• W2021777797 cites W1971908211 @default.
• W2021777797 cites W1974159422 @default.
• W2021777797 cites W1980119013 @default.
• W2021777797 cites W1983224547 @default.
• W2021777797 cites W1991891818 @default.
• W2021777797 cites W1994507627 @default.
• W2021777797 cites W1996548067 @default.
• W2021777797 cites W1999028040 @default.
• W2021777797 cites W2021297146 @default.
• W2021777797 cites W2022123949 @default.
• W2021777797 cites W2024893080 @default.
• W2021777797 cites W2034277598 @default.
• W2021777797 cites W2046396043 @default.
• W2021777797 cites W2049917390 @default.
• W2021777797 cites W2071061915 @default.
• W2021777797 cites W2076182160 @default.
• W2021777797 cites W2087639358 @default.
• W2021777797 cites W2094111001 @default.
• W2021777797 cites W2097520274 @default.
• W2021777797 cites W2100837269 @default.
• W2021777797 cites W2101331563 @default.
• W2021777797 cites W2115798976 @default.
• W2021777797 cites W2120486924 @default.
• W2021777797 cites W2166670696 @default.
• W2021777797 cites W2203821910 @default.
• W2021777797 cites W4244158828 @default.
• W2021777797 doi "https://doi.org/10.1111/j.1432-1033.1996.00837.x" @default.
• W2021777797 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/8665902" @default.
• W2021777797 hasPublicationYear "1996" @default.
• W2021777797 type Work @default.
• W2021777797 sameAs 2021777797 @default.
• W2021777797 citedByCount "65" @default.
• W2021777797 countsByYear W20217777972012 @default.
• W2021777797 countsByYear W20217777972013 @default.
• W2021777797 countsByYear W20217777972014 @default.
• W2021777797 countsByYear W20217777972015 @default.
• W2021777797 countsByYear W20217777972016 @default.
• W2021777797 countsByYear W20217777972017 @default.
• W2021777797 countsByYear W20217777972018 @default.
• W2021777797 countsByYear W20217777972019 @default.
• W2021777797 countsByYear W20217777972020 @default.
• W2021777797 countsByYear W20217777972022 @default.
• W2021777797 countsByYear W20217777972023 @default.
• W2021777797 crossrefType "journal-article" @default.
• W2021777797 hasAuthorship W2021777797A5016159915 @default.
• W2021777797 hasAuthorship W2021777797A5025374231 @default.
• W2021777797 hasAuthorship W2021777797A5027069639 @default.
• W2021777797 hasAuthorship W2021777797A5028612887 @default.
• W2021777797 hasAuthorship W2021777797A5036852859 @default.
• W2021777797 hasAuthorship W2021777797A5038296391 @default.
• W2021777797 hasAuthorship W2021777797A5054786640 @default.
• W2021777797 hasAuthorship W2021777797A5075814041 @default.
• W2021777797 hasAuthorship W2021777797A5079991502 @default.
• W2021777797 hasBestOaLocation W20217777971 @default.
• W2021777797 hasConcept C104317684 @default.
• W2021777797 hasConcept C153911025 @default.
• W2021777797 hasConcept C167625842 @default.
• W2021777797 hasConcept C170493617 @default.
• W2021777797 hasConcept C179247698 @default.
• W2021777797 hasConcept C181199279 @default.
• W2021777797 hasConcept C185592680 @default.
• W2021777797 hasConcept C194583182 @default.
• W2021777797 hasConcept C2779281246 @default.
• W2021777797 hasConcept C53345823 @default.
• W2021777797 hasConcept C55493867 @default.
• W2021777797 hasConcept C58121356 @default.
• W2021777797 hasConcept C86803240 @default.
• W2021777797 hasConceptScore W2021777797C104317684 @default.
• W2021777797 hasConceptScore W2021777797C153911025 @default.
• W2021777797 hasConceptScore W2021777797C167625842 @default.
• W2021777797 hasConceptScore W2021777797C170493617 @default.
• W2021777797 hasConceptScore W2021777797C179247698 @default.
• W2021777797 hasConceptScore W2021777797C181199279 @default.
• W2021777797 hasConceptScore W2021777797C185592680 @default.
• W2021777797 hasConceptScore W2021777797C194583182 @default.

• next