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• W2021813100 abstract "Abstract We have obtained Nicotiana tabacum transgenic cell lines expressing a sucrose binding protein ( sbp ) homologue gene from soybean ( Glycine max L.), designated s-64 , either in the sense or antisense orientation. Sense cell lines over-accumulated the S-64 protein, whereas the antisense cell lines had reduced levels of the endogenous homologue protein. Sucrose uptake experiments were conducted by incubating suspension-cultured tobacco cells with radiolabeled sucrose at pH 4.5 or 7.0. Raising the extracellular pH to 7.0 caused an inhibition of radiolabeled carbon uptake efficiency, which was attributed to the pH-sensitivity of cell-wall invertase (EC 3.2.1.26), H + /hexose transporter and/or H + /sucrose symporter activities. Because SBP-mediated sucrose uptake has been shown to be insensitive to extracellular pH in yeast, we performed the sucrose uptake experiments in sense and antisense cultured cells at pH 7.0. Under this condition, the level of SBP homologue correlated with the efficiency of radiolabeled uptake by the transgenic tobacco cells. Furthermore, manipulation of S-64 levels altered sucrose-cleaving activities in a metabolic compensatory manner. Enhanced accumulation of S-64 caused an increase in intracellular sucrose synthase (cleavage, EC 2.4.1.13) activity with a concomitant decline in cell-wall invertase activity. This result may reflect a metabolic adjustment of the sense cell lines caused by its high efficiency of direct sucrose uptake as disaccharide. In contrast, the level of cell-wall invertase activity was remarkably increased in antisense cells, favoring the invertase-dependent sugar uptake system. Collectively, these results may establish a functional link between radiolabeled influx and S-64 accumulation, suggesting that SBP affects sucrose uptake in suspension-cultured cells." @default.
• W2021813100 created "2016-06-24" @default.
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• W2021813100 date "2000-05-01" @default.
• W2021813100 modified "2023-09-27" @default.
• W2021813100 title "A sucrose binding protein homologue from soybean affects sucrose uptake in suspension-cultured transgenic tobacco cells" @default.
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• W2021813100 doi "https://doi.org/10.1016/s0981-9428(00)00752-x" @default.
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