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- W2021832728 abstract "Abstract Two equine infectious anemia virus complementary DNA probes synthesized using detergent-treated purified virus were used to analyze the integrated provirus of persistently infected equine dermal cells. The probes were (i) cDNAd 5′ , an endogenously primed unique sequence duplex transcript representative of ∼15% of genomic RNA and enriched in sequences represented in randomly fragmented viral RNA not retained by oligo(dT)-cellulose and (ii) 6DNA rep , a calf thymus DNA-primed single-stranded transcript symmetrically representative of 80–85% of genomic RNA. Integrated provirus homologous to cDNA 5′ , is present with a repetitive frequency on the order of 100 copies per cell whereas provirus homologous to cDNA rep is integrated with a frequency of only two to six copies. The cell cultures are uniformly infected as measured by detection of viral RNA by in situ hybridization, and integrated provirus is associated with high-molecular-weight cell DNA by alkali-stable linkage. Provirus is not detectable in uninfected cells." @default.
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- W2021832728 date "1982-04-01" @default.
- W2021832728 modified "2023-09-24" @default.
- W2021832728 title "Persistent infection by equine infectious anemia virus: Asymmetry of nucleotide sequence reiteration in the integrated provirus of persistently infected cells" @default.
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- W2021832728 doi "https://doi.org/10.1016/0042-6822(82)90340-3" @default.
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