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• W2021851982 abstract "Manduca sexta allatotropin (Manse-AT) was first isolated on the basis of its ability to stimulate production of juvenile hormone in that insect. We examined whether this neuropeptide affects corpus allatum activity and visceral muscle contraction in adult females of the earwig, Euborellia annulipes. We also assessed the presence of allatotropin-like material in tissues using immunocytochemistry. Manse-AT at 1 nM to 10 muM stimulated juvenile hormone production in vitro by glands of low activity from 2-day virgin females. In glands of high activity from 12-day mated females, 1 and 100 nM allatotropin were effective, but 10 muM was not. Similarly, hindguts of 2-day and 12-day females significantly increased in motility in vitro in response to Manse-AT. A monoclonal antibody to Manse-AT was used to demonstrate allatotropin-like material throughout the nervous system of 2-day, virgin females. Immunoreactivity was most pronounced within varicosities of the corpora cardiaca and perisympathetic organs. No immunofluorescence was observed in gut tissue. Lastly, we showed that extract of retrocerebral complexes also enhanced in vitro hindgut motility from 2-day virgin females, in a dose-dependent manner. These results indicate material similar to M. sexta allatotropin in female earwigs and that such peptides may modulate juvenile hormone biosynthesis and visceral muscle contractions. Sensitivity to the peptides may change with physiological stage." @default.
• W2021851982 created "2016-06-24" @default.
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• W2021851982 date "2005-09-01" @default.
• W2021851982 modified "2023-09-27" @default.
• W2021851982 title "Effects of Manduca allatotropin and localization of Manduca allatotropin-immunoreactive cells in earwigs" @default.
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• W2021851982 doi "https://doi.org/10.1016/j.cbpc.2005.06.006" @default.
• W2021851982 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/16019245" @default.
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