FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2021852783> ?p ?o ?g. }

• W2021852783 endingPage "e24269" @default.
• W2021852783 startingPage "e24269" @default.
• W2021852783 abstract "Objectives Genetic defects leading to the reduction of the survival motor neuron protein (SMN) are a causal factor for Spinal Muscular Atrophy (SMA). While there are a number of therapies under evaluation as potential treatments for SMA, there is a critical lack of a biomarker method for assessing efficacy of therapeutic interventions, particularly those targeting upregulation of SMN protein levels. Towards this end we have engaged in developing an immunoassay capable of accurately measuring SMN protein levels in blood, specifically in peripheral blood mononuclear cells (PBMCs), as a tool for validating SMN protein as a biomarker in SMA. Methods A sandwich enzyme-linked immunosorbent assay (ELISA) was developed and validated for measuring SMN protein in human PBMCs and other cell lysates. Protocols for detection and extraction of SMN from transgenic SMA mouse tissues were also developed. Results The assay sensitivity for human SMN is 50 pg/mL. Initial analysis reveals that PBMCs yield enough SMN to analyze from blood volumes of less than 1 mL, and SMA Type I patients' PBMCs show ∼90% reduction of SMN protein compared to normal adults. The ELISA can reliably quantify SMN protein in human and mouse PBMCs and muscle, as well as brain, and spinal cord from a mouse model of severe SMA. Conclusions This SMN ELISA assay enables the reliable, quantitative and rapid measurement of SMN in healthy human and SMA patient PBMCs, muscle and fibroblasts. SMN was also detected in several tissues in a mouse model of SMA, as well as in wildtype mouse tissues. This SMN ELISA has general translational applicability to both preclinical and clinical research efforts." @default.
• W2021852783 created "2016-06-24" @default.
• W2021852783 creator A5026336452 @default.
• W2021852783 creator A5026563103 @default.
• W2021852783 creator A5026734571 @default.
• W2021852783 creator A5031572739 @default.
• W2021852783 creator A5033860761 @default.
• W2021852783 creator A5036479757 @default.
• W2021852783 creator A5045954335 @default.
• W2021852783 creator A5062118853 @default.
• W2021852783 creator A5075884817 @default.
• W2021852783 creator A5083074546 @default.
• W2021852783 date "2011-08-31" @default.
• W2021852783 modified "2023-10-11" @default.
• W2021852783 title "Utility of Survival Motor Neuron ELISA for Spinal Muscular Atrophy Clinical and Preclinical Analyses" @default.
• W2021852783 cites W1496859054 @default.
• W2021852783 cites W1965284203 @default.
• W2021852783 cites W1968847496 @default.
• W2021852783 cites W1971203455 @default.
• W2021852783 cites W1980477780 @default.
• W2021852783 cites W1987687980 @default.
• W2021852783 cites W1989394723 @default.
• W2021852783 cites W1996412713 @default.
• W2021852783 cites W1999759329 @default.
• W2021852783 cites W2002912370 @default.
• W2021852783 cites W2006264599 @default.
• W2021852783 cites W2008333108 @default.
• W2021852783 cites W2008834682 @default.
• W2021852783 cites W2020999287 @default.
• W2021852783 cites W2024736109 @default.
• W2021852783 cites W2025722577 @default.
• W2021852783 cites W2026646891 @default.
• W2021852783 cites W2035461158 @default.
• W2021852783 cites W2037353151 @default.
• W2021852783 cites W2045117117 @default.
• W2021852783 cites W2054380003 @default.
• W2021852783 cites W2061972906 @default.
• W2021852783 cites W2067161603 @default.
• W2021852783 cites W2070086105 @default.
• W2021852783 cites W2080464008 @default.
• W2021852783 cites W2088820444 @default.
• W2021852783 cites W2094072263 @default.
• W2021852783 cites W2101481997 @default.
• W2021852783 cites W2104483488 @default.
• W2021852783 cites W2105059456 @default.
• W2021852783 cites W2108430210 @default.
• W2021852783 cites W2111871851 @default.
• W2021852783 cites W2118374730 @default.
• W2021852783 cites W2119435334 @default.
• W2021852783 cites W2122420087 @default.
• W2021852783 cites W2124384944 @default.
• W2021852783 cites W2129447667 @default.
• W2021852783 cites W2135399845 @default.
• W2021852783 cites W2136868631 @default.
• W2021852783 cites W2138675678 @default.
• W2021852783 cites W2141047754 @default.
• W2021852783 cites W2146223217 @default.
• W2021852783 cites W2148536601 @default.
• W2021852783 cites W2151318390 @default.
• W2021852783 cites W2151538140 @default.
• W2021852783 cites W2152766938 @default.
• W2021852783 cites W2158920269 @default.
• W2021852783 cites W2165235225 @default.
• W2021852783 cites W2170003503 @default.
• W2021852783 cites W2171997474 @default.
• W2021852783 cites W2172093090 @default.
• W2021852783 doi "https://doi.org/10.1371/journal.pone.0024269" @default.
• W2021852783 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3164180" @default.
• W2021852783 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/21904622" @default.
• W2021852783 hasPublicationYear "2011" @default.
• W2021852783 type Work @default.
• W2021852783 sameAs 2021852783 @default.
• W2021852783 citedByCount "25" @default.
• W2021852783 countsByYear W20218527832012 @default.
• W2021852783 countsByYear W20218527832013 @default.
• W2021852783 countsByYear W20218527832014 @default.
• W2021852783 countsByYear W20218527832015 @default.
• W2021852783 countsByYear W20218527832016 @default.
• W2021852783 countsByYear W20218527832017 @default.
• W2021852783 countsByYear W20218527832018 @default.
• W2021852783 countsByYear W20218527832019 @default.
• W2021852783 countsByYear W20218527832020 @default.
• W2021852783 countsByYear W20218527832021 @default.
• W2021852783 countsByYear W20218527832022 @default.
• W2021852783 countsByYear W20218527832023 @default.
• W2021852783 crossrefType "journal-article" @default.
• W2021852783 hasAuthorship W2021852783A5026336452 @default.
• W2021852783 hasAuthorship W2021852783A5026563103 @default.
• W2021852783 hasAuthorship W2021852783A5026734571 @default.
• W2021852783 hasAuthorship W2021852783A5031572739 @default.
• W2021852783 hasAuthorship W2021852783A5033860761 @default.
• W2021852783 hasAuthorship W2021852783A5036479757 @default.
• W2021852783 hasAuthorship W2021852783A5045954335 @default.
• W2021852783 hasAuthorship W2021852783A5062118853 @default.
• W2021852783 hasAuthorship W2021852783A5075884817 @default.
• W2021852783 hasAuthorship W2021852783A5083074546 @default.
• W2021852783 hasBestOaLocation W20218527831 @default.
• W2021852783 hasConcept C114614502 @default.

• next