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• W2021866639 endingPage "2014.e10" @default.
• W2021866639 startingPage "2014.e7" @default.
• W2021866639 abstract "To report a live birth after the transfer of a single blastocyst derived from a human oocyte cryopreserved for 5 years.Case report.Private assisted reproduction center.A 39-year-old woman with tubal infertility and her partner with male-related infertility.Oocyte cryopreservation (in 1.5 mol/L 1,2-propanediol and 0.3 mol/L sucrose by slow freezing-rapid thawing protocol) when the patient was 34 years old. Oocyte thawing after 5 years of cryostorage. Insemination by intracytoplasmic sperm injection of the three best surviving oocytes, according to the Italian law regulating assisted reproductive technology.Oocyte survival after thawing. Fertilization, cleavage, and embryo development into blastocyst stage.Four of six mature (metaphase II) frozen oocytes survived after thawing. Three of them were injected: these three oocytes were fertilized, and one developed into a five-cell embryo on day 3. This embryo developed into blastocyst and was transferred on day 6. A healthy female neonate weighing 3,410 g was born.After 5 years of storage in liquid nitrogen, cryopreserved oocytes with a slow cooling-rapid thawing protocol can develop in vitro to blastocyst stage and produce a live birth." @default.
• W2021866639 created "2016-06-24" @default.
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• W2021866639 date "2008-11-01" @default.
• W2021866639 modified "2023-10-01" @default.
• W2021866639 title "Blastocyst formation, pregnancy, and birth derived from human oocytes cryopreserved for 5 years" @default.
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• W2021866639 doi "https://doi.org/10.1016/j.fertnstert.2008.01.106" @default.
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