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• W2021879931 abstract "To clarify the profiles of two distinct gonadotropin (GTH-I and -II) mRNA levels during gametogenesis in a multiple spawner, the Japanese flounder (Paralichthys olivaceus), the cDNAs encoding GTH-Ibeta and -IIbeta from the pituitary gland have been cloned and sequenced. The nucleotide sequence of GTH-Ibeta was 542 bp long, encoding 120 amino acids, and that of GTH-IIbeta was 554 bp long, encoding 145 amino acids. In females, Northern blot analysis has revealed that relative mRNA levels of GTH-Ibeta and -IIbeta were low in immature fish, showed a gradual increase with ovarian development, and reached the highest level at the maturation stage. Both GTH-Ibeta and -IIbeta mRNA levels were highly correlated with gonadosomatic index (GSI) values and with circulating estradiol-17beta and testosterone (T) levels. In males, the mRNA levels of GTH-Ibeta increased with the increase in GSI values and in circulating 11-ketotestosterone and T levels, whereas the mRNA levels of GTH-IIbeta did not show any correlation with GSI values and with circulating steroid levels, suggesting a difference in regulatory mechanisms of GTH-I and -II synthesis in males. The similar changes in GTH-Ibeta and -IIbeta mRNA levels during oogenesis are considered to be characteristic of GTH synthesis in multiple spawners, differing from the differential changes reported in annual spawners such as salmonids." @default.
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• W2021879931 date "2001-05-01" @default.
• W2021879931 modified "2023-10-04" @default.
• W2021879931 title "cDNA Cloning of Two Gonadotropin β Subunits (GTH-Iβ and -IIβ) and Their Expression Profiles during Gametogenesis in the Japanese Flounder (Paralichthys olivaceus)" @default.
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• W2021879931 doi "https://doi.org/10.1006/gcen.2000.7610" @default.
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