FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2021884639> ?p ?o ?g. }

• W2021884639 endingPage "4431" @default.
• W2021884639 startingPage "4422" @default.
• W2021884639 abstract "Pregnancy-specific glycoproteins (PSGs) are a family of Ig-like proteins secreted by specialized placental cells. The PSG1 structure is composed of a single Ig variable region-like N-terminal domain and three Ig constant region-like domains termed A1, A2, and B2. Members of the human and murine PSG family have been shown to induce anti-inflammatory cytokines from monocytes and macrophages and to stimulate angiogenesis. We recently showed that recombinant forms of PSG1 (PSG1-Fc and PSG1-His) and PSG1 purified from the serum of pregnant women are associated with the immunoregulatory cytokine TGF-β1 and activated latent TGF-β1. Here, we sought to examine the requirement of specific PSG1 domains in the activation of latent TGF-β1. Plasmon surface resonance studies showed that PSG1 directly bound to the small latent complex and to the latency-associated peptide of TGF-β1 and that this binding was mediated through the B2 domain. Furthermore, the B2 domain alone was sufficient for activating the small latent complex. In separate experiments, we found that the PSG1-mediated induction of TGF-β1 secretion in macrophages was dependent on the N-terminal domain. Mutagenesis analysis revealed that four amino acids (LYHY) of the CC′ loop of the N-terminal domain were required for induction of latent TGF-β1 secretion. Together, our results show that two distinct domains of PSG1 are involved in the regulation of TGF-β1 and provide a mechanistic framework for how PSGs modulate the immunoregulatory environment at the maternal-fetal interface for successful pregnancy outcome. Pregnancy-specific glycoproteins (PSGs) are a family of Ig-like proteins secreted by specialized placental cells. The PSG1 structure is composed of a single Ig variable region-like N-terminal domain and three Ig constant region-like domains termed A1, A2, and B2. Members of the human and murine PSG family have been shown to induce anti-inflammatory cytokines from monocytes and macrophages and to stimulate angiogenesis. We recently showed that recombinant forms of PSG1 (PSG1-Fc and PSG1-His) and PSG1 purified from the serum of pregnant women are associated with the immunoregulatory cytokine TGF-β1 and activated latent TGF-β1. Here, we sought to examine the requirement of specific PSG1 domains in the activation of latent TGF-β1. Plasmon surface resonance studies showed that PSG1 directly bound to the small latent complex and to the latency-associated peptide of TGF-β1 and that this binding was mediated through the B2 domain. Furthermore, the B2 domain alone was sufficient for activating the small latent complex. In separate experiments, we found that the PSG1-mediated induction of TGF-β1 secretion in macrophages was dependent on the N-terminal domain. Mutagenesis analysis revealed that four amino acids (LYHY) of the CC′ loop of the N-terminal domain were required for induction of latent TGF-β1 secretion. Together, our results show that two distinct domains of PSG1 are involved in the regulation of TGF-β1 and provide a mechanistic framework for how PSGs modulate the immunoregulatory environment at the maternal-fetal interface for successful pregnancy outcome." @default.
• W2021884639 created "2016-06-24" @default.
• W2021884639 creator A5006885476 @default.
• W2021884639 creator A5020046699 @default.
• W2021884639 creator A5045077371 @default.
• W2021884639 creator A5068299120 @default.
• W2021884639 creator A5087492882 @default.
• W2021884639 date "2015-02-01" @default.
• W2021884639 modified "2023-10-18" @default.
• W2021884639 title "Induction and Activation of Latent Transforming Growth Factor-β1 Are Carried out by Two Distinct Domains of Pregnancy-specific Glycoprotein 1 (PSG1)" @default.
• W2021884639 cites W1556193995 @default.
• W2021884639 cites W1575754639 @default.
• W2021884639 cites W1670146094 @default.
• W2021884639 cites W1904531954 @default.
• W2021884639 cites W1913128941 @default.
• W2021884639 cites W1970455281 @default.
• W2021884639 cites W1974985218 @default.
• W2021884639 cites W1978202313 @default.
• W2021884639 cites W1986191025 @default.
• W2021884639 cites W1994285497 @default.
• W2021884639 cites W2012402693 @default.
• W2021884639 cites W2018265937 @default.
• W2021884639 cites W2018822666 @default.
• W2021884639 cites W2020722973 @default.
• W2021884639 cites W2024197161 @default.
• W2021884639 cites W2025406290 @default.
• W2021884639 cites W2031131334 @default.
• W2021884639 cites W2033260397 @default.
• W2021884639 cites W2041801047 @default.
• W2021884639 cites W2043517504 @default.
• W2021884639 cites W2051816002 @default.
• W2021884639 cites W2054187879 @default.
• W2021884639 cites W2065955846 @default.
• W2021884639 cites W2078420687 @default.
• W2021884639 cites W2081508441 @default.
• W2021884639 cites W2090043394 @default.
• W2021884639 cites W2095537212 @default.
• W2021884639 cites W2100314344 @default.
• W2021884639 cites W2100358681 @default.
• W2021884639 cites W2107492957 @default.
• W2021884639 cites W2113313714 @default.
• W2021884639 cites W2120449802 @default.
• W2021884639 cites W2122931228 @default.
• W2021884639 cites W2129434797 @default.
• W2021884639 cites W2148115602 @default.
• W2021884639 cites W2152301430 @default.
• W2021884639 cites W2158562723 @default.
• W2021884639 cites W2164506059 @default.
• W2021884639 doi "https://doi.org/10.1074/jbc.m114.597518" @default.
• W2021884639 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4326847" @default.
• W2021884639 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/25548275" @default.
• W2021884639 hasPublicationYear "2015" @default.
• W2021884639 type Work @default.
• W2021884639 sameAs 2021884639 @default.
• W2021884639 citedByCount "31" @default.
• W2021884639 countsByYear W20218846392015 @default.
• W2021884639 countsByYear W20218846392016 @default.
• W2021884639 countsByYear W20218846392017 @default.
• W2021884639 countsByYear W20218846392018 @default.
• W2021884639 countsByYear W20218846392019 @default.
• W2021884639 countsByYear W20218846392020 @default.
• W2021884639 countsByYear W20218846392021 @default.
• W2021884639 countsByYear W20218846392022 @default.
• W2021884639 countsByYear W20218846392023 @default.
• W2021884639 crossrefType "journal-article" @default.
• W2021884639 hasAuthorship W2021884639A5006885476 @default.
• W2021884639 hasAuthorship W2021884639A5020046699 @default.
• W2021884639 hasAuthorship W2021884639A5045077371 @default.
• W2021884639 hasAuthorship W2021884639A5068299120 @default.
• W2021884639 hasAuthorship W2021884639A5087492882 @default.
• W2021884639 hasBestOaLocation W20218846391 @default.
• W2021884639 hasConcept C108625454 @default.
• W2021884639 hasConcept C118131993 @default.
• W2021884639 hasConcept C153911025 @default.
• W2021884639 hasConcept C185592680 @default.
• W2021884639 hasConcept C203014093 @default.
• W2021884639 hasConcept C2778690821 @default.
• W2021884639 hasConcept C49039625 @default.
• W2021884639 hasConcept C55493867 @default.
• W2021884639 hasConcept C86803240 @default.
• W2021884639 hasConcept C95444343 @default.
• W2021884639 hasConceptScore W2021884639C108625454 @default.
• W2021884639 hasConceptScore W2021884639C118131993 @default.
• W2021884639 hasConceptScore W2021884639C153911025 @default.
• W2021884639 hasConceptScore W2021884639C185592680 @default.
• W2021884639 hasConceptScore W2021884639C203014093 @default.
• W2021884639 hasConceptScore W2021884639C2778690821 @default.
• W2021884639 hasConceptScore W2021884639C49039625 @default.
• W2021884639 hasConceptScore W2021884639C55493867 @default.
• W2021884639 hasConceptScore W2021884639C86803240 @default.
• W2021884639 hasConceptScore W2021884639C95444343 @default.
• W2021884639 hasFunder F4320332161 @default.
• W2021884639 hasIssue "7" @default.
• W2021884639 hasLocation W20218846391 @default.
• W2021884639 hasLocation W20218846392 @default.
• W2021884639 hasLocation W20218846393 @default.
• W2021884639 hasOpenAccess W2021884639 @default.
• W2021884639 hasPrimaryLocation W20218846391 @default.

• next