FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2021970375> ?p ?o ?g. }

• W2021970375 endingPage "1591" @default.
• W2021970375 startingPage "1578" @default.
• W2021970375 abstract "Heat-shock protein 90 (Hsp90) has a crucial role in both the stabilisation and regulation of various proteins, including those related to radioresistance. Inhibition of Hsp90 may therefore provide a strategy for enhancing the radiosensitivity of tumour cells. This study explores the responses of four tumour cell lines (A549, GaMG, HT 1080 and SNB19) to combined treatment with ionising radiation (IR) and two novel inhibitors of Hsp90, NVP-AUY922 and NVP-BEP800. The techniques used included cell and colony counts, expression of Hsp90, Hsp70, Akt, survivin, cleaved caspase 3, p53, cell-cycle progression and associated proteins. DNA damage was analysed by histone γH2AX and Comet assays. We found that NVP-AUY922 and NVP-BEP800 enhanced radiosensitivity in all tested cell lines. In contrast, only two cell lines (HT 1080 and GaMG) exhibited an increased rate of apoptosis after drug pretreatment, as revealed by western blot. In all tested cell lines, the expression of histone γH2AX, a marker of DNA double-strand breaks, after combined drug-IR treatment was higher and its decay rate was slower than those after each single treatment modality. Drug-IR treatment also resulted in impaired cell-cycle progression, as indicated by S-phase depletion and G2/M arrest. In addition, the cell cycle-associated proteins, Cdk1 and Cdk4, were downregulated after Hsp90 inhibition. These findings show that the novel inhibitors of Hsp90 can radiosensitise tumour cell lines of different entities through destabilisation and depletion of several Hsp90 client proteins, thus causing the depletion of S phase and G2/M arrest, increased DNA damage and repair protraction and, to some extent, apoptosis. The results might have important implications for the radiotherapy of solid tumours." @default.
• W2021970375 created "2016-06-24" @default.
• W2021970375 creator A5009855159 @default.
• W2021970375 creator A5018379301 @default.
• W2021970375 creator A5029953314 @default.
• W2021970375 creator A5046756579 @default.
• W2021970375 creator A5080827305 @default.
• W2021970375 creator A5084766894 @default.
• W2021970375 date "2010-05-01" @default.
• W2021970375 modified "2023-10-17" @default.
• W2021970375 title "Novel HSP90 inhibitors, NVP-AUY922 and NVP-BEP800, radiosensitise tumour cells through cell-cycle impairment, increased DNA damage and repair protraction" @default.
• W2021970375 cites W1967144774 @default.
• W2021970375 cites W1978356732 @default.
• W2021970375 cites W1979397363 @default.
• W2021970375 cites W1982516207 @default.
• W2021970375 cites W1982810405 @default.
• W2021970375 cites W1985775217 @default.
• W2021970375 cites W1986139683 @default.
• W2021970375 cites W1986242330 @default.
• W2021970375 cites W1998071923 @default.
• W2021970375 cites W1999715990 @default.
• W2021970375 cites W2000101754 @default.
• W2021970375 cites W2000700488 @default.
• W2021970375 cites W2004792969 @default.
• W2021970375 cites W2013117207 @default.
• W2021970375 cites W2015181794 @default.
• W2021970375 cites W2022531767 @default.
• W2021970375 cites W2028978816 @default.
• W2021970375 cites W2029212618 @default.
• W2021970375 cites W2034135451 @default.
• W2021970375 cites W2040235808 @default.
• W2021970375 cites W2049562411 @default.
• W2021970375 cites W2057574712 @default.
• W2021970375 cites W2074579328 @default.
• W2021970375 cites W2075805324 @default.
• W2021970375 cites W2076981817 @default.
• W2021970375 cites W2088929311 @default.
• W2021970375 cites W2095403735 @default.
• W2021970375 cites W2101100014 @default.
• W2021970375 cites W2103618960 @default.
• W2021970375 cites W2107831273 @default.
• W2021970375 cites W2109635468 @default.
• W2021970375 cites W2137911213 @default.
• W2021970375 cites W2151908249 @default.
• W2021970375 cites W2152649141 @default.
• W2021970375 cites W2162107265 @default.
• W2021970375 cites W2165088901 @default.
• W2021970375 cites W2166999544 @default.
• W2021970375 cites W2167356883 @default.
• W2021970375 cites W2168434477 @default.
• W2021970375 cites W4243574300 @default.
• W2021970375 doi "https://doi.org/10.1038/sj.bjc.6605683" @default.
• W2021970375 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/2883148" @default.
• W2021970375 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/20502461" @default.
• W2021970375 hasPublicationYear "2010" @default.
• W2021970375 type Work @default.
• W2021970375 sameAs 2021970375 @default.
• W2021970375 citedByCount "76" @default.
• W2021970375 countsByYear W20219703752012 @default.
• W2021970375 countsByYear W20219703752013 @default.
• W2021970375 countsByYear W20219703752014 @default.
• W2021970375 countsByYear W20219703752015 @default.
• W2021970375 countsByYear W20219703752016 @default.
• W2021970375 countsByYear W20219703752017 @default.
• W2021970375 countsByYear W20219703752018 @default.
• W2021970375 countsByYear W20219703752019 @default.
• W2021970375 countsByYear W20219703752020 @default.
• W2021970375 countsByYear W20219703752021 @default.
• W2021970375 countsByYear W20219703752022 @default.
• W2021970375 crossrefType "journal-article" @default.
• W2021970375 hasAuthorship W2021970375A5009855159 @default.
• W2021970375 hasAuthorship W2021970375A5018379301 @default.
• W2021970375 hasAuthorship W2021970375A5029953314 @default.
• W2021970375 hasAuthorship W2021970375A5046756579 @default.
• W2021970375 hasAuthorship W2021970375A5080827305 @default.
• W2021970375 hasAuthorship W2021970375A5084766894 @default.
• W2021970375 hasBestOaLocation W20219703751 @default.
• W2021970375 hasConcept C104317684 @default.
• W2021970375 hasConcept C105696609 @default.
• W2021970375 hasConcept C120504264 @default.
• W2021970375 hasConcept C126322002 @default.
• W2021970375 hasConcept C134935766 @default.
• W2021970375 hasConcept C143425029 @default.
• W2021970375 hasConcept C153911025 @default.
• W2021970375 hasConcept C190283241 @default.
• W2021970375 hasConcept C205260736 @default.
• W2021970375 hasConcept C206684579 @default.
• W2021970375 hasConcept C2780358455 @default.
• W2021970375 hasConcept C29537977 @default.
• W2021970375 hasConcept C502942594 @default.
• W2021970375 hasConcept C509974204 @default.
• W2021970375 hasConcept C54355233 @default.
• W2021970375 hasConcept C552990157 @default.
• W2021970375 hasConcept C55493867 @default.
• W2021970375 hasConcept C58962609 @default.
• W2021970375 hasConcept C71924100 @default.
• W2021970375 hasConcept C81885089 @default.
• W2021970375 hasConcept C86803240 @default.

• next