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- W2021982661 abstract "Background: Glutathione S-transferases (GSTs) contribute to liver homeostasis and are released into plasma after liver injury. This study investigates the hepatic expression of key GST enzyme subtypes after burn injury. Methods: Mice were subjected to an 18% total body surface area burn. Expression of mGSTα3 and mGSTμ1 was analyzed by reverse-transcriptase polymerase chain reaction and Western blots on liver preparations from burned and control mice. GST catalytic activity was measured by conjugation assays. Results: In burned animals, mGSTα3 mRNA was only reduced at 1 day, whereas mGSTμ1 mRNA decreased at 1 day and at 3 days after injury. Total mGSTμ protein levels decreased at the same time points, whereas total mGSTα protein levels did not change. Also, by 3 days, total GST conjugation activity was reduced by 25% (p = 0.05). Conclusion: Reduced expression of specific α and μ GST enzymes after burns correlates functionally with reduced GST activity. Altered GST expression may contribute to liver damage after systemic injury." @default.
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- W2021982661 date "2004-10-01" @default.
- W2021982661 modified "2023-09-27" @default.
- W2021982661 title "Reduced Hepatic Expression of Glutathione S-Transferases in Burned Mice" @default.
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- W2021982661 doi "https://doi.org/10.1097/01.ta.0000096641.32787.3f" @default.
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