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- W2021984472 abstract "We prepared a rabbit polyclonal antibody against an endothelin converting enzyme-1 (ECE-1) to examine the distribution of ECE-1 in the hypothalamus and the pituitary of the rat. By the Western blot analysis, the antibody specifically recognized ECE-1 proteins in membrane fractions of the rat hypothalamus. Immunohistochemical staining revealed ECE-1 immunoreactivity in both cell bodies and axons in some hypothalamic neurons. The immunopositive cell bodies were located in the paraventricullar and supraoptic nuclei; immunopositive axons were distributed in the suprachiasmatic, paraventricullar (PVN), and supraoptic (SON) nuclei. However no immunoreactivity was detected in the posterior pituitary gland. The present results indicate that ECE-1 converts the endothelin-1 (ET-1) from precursor big ET-1 to mature ET-1 in the PVN/SON. The big ET-1 existing in the posterior pituitary gland is thought to be escaped from the converting by ECE-1 in the hypothalamus." @default.
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- W2021984472 date "2000-04-01" @default.
- W2021984472 modified "2023-09-23" @default.
- W2021984472 title "Immunohistochemical distribution of the endothelin-converting enzyme-1 in the rat hypothalamo-pituitary axis" @default.
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- W2021984472 doi "https://doi.org/10.1016/s0304-3940(00)00974-5" @default.
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