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• W2021990228 abstract "Ro autoantibodies, including Ro52, have been detected in the sera of patients with Sjögren’s syndrome and lupus erythematosus. In patients with lupus, Ro autoantibodies have been associated with photosensitivity and with the lupus subtype subacute cutaneous lupus erythematosus (Sontheimer et al., 1982Sontheimer R.D. Maddison P.J. Reichlin M. Jordon R.E. Stastny P. Gilliam J.N. Serologic and HLA associations in subacute cutaneous lupus erythematosus, a clinical subset of lupus erythematosus.Ann Intern Med. 1982; 97: 664-671Crossref PubMed Scopus (331) Google Scholar). Tissue from patients with lupus exhibits Ro52 staining in mononuclear cells, especially B cells and those with antiproliferative and cell death–sensitizing properties (Espinosa et al., 2006Espinosa A. Zhou W. Ek M. Hedlund M. Brauner S. Popovic K. et al.The Sjögren’s syndrome-associated autoantigen Ro52 is an E3 ligase that regulates proliferation and cell death.J Immunol. 2006; 176: 6277-6285Crossref PubMed Scopus (121) Google Scholar). Ro52 may be a factor in other inflammatory processes as well (Higgs et al., 2008Higgs R. Ni Gabhann J. Ben Larbi N. Breen E.P. Fitzgerald K.A. Jefferies C.A. The E3 ubiquitin ligase Ro52 negatively regulates IFN-beta production post-pathogen recognition by polyubiquitin-mediated degradation of IRF3.J Immunol. 2008; 181: 1780-1786Crossref PubMed Scopus (206) Google Scholar), but the ability to detect Ro52 in tissue has been limited by the lack of suitable monoclonal antibodies for immunohistochemistry. In this study, Oke et al., 2009Oke V. Vassilaki I. Espinosa A. Strandberg L. Kuchroo V.K. Nyberg F. et al.High Ro52 expression in spontaneous and UV-induced cutaneous inflammation.J Invest Dermatol. 2009; 129: 2000-2010Abstract Full Text Full Text PDF PubMed Scopus (45) Google Scholar developed a panel of Ro52 monoclonal antibodies to study the presence and patterns of Ro52 expression. They initially tested four monoclonal antibodies in both frozen and paraffin-embedded tissues. These experiments all yielded similar results, and a single antibody (7.8C7) and paraffin-embedded tissues were chosen for subsequent experiments. Using this monoclonal antibody, Oke et al. studied Ro52 expression in lesional and nonlesional skin from patients with lupus and from healthy control subjects, as well as in UVR-induced lesions from patients and in UVR-provoked skin from healthy controls. Experiments were confirmed in cultured keratinocytes from the nonlesional skin of patients and healthy controls. They demonstrated high expression of Ro52 in the cytoplasm of keratinocytes in samples from patients with cutaneous lupus compared with that of controls. Ro52 antigen was found to be upregulated by UVR in photoprovocation testing. In vitro confirmatory experiments using cultured keratinocytes exposed to UV radiation resulted in increased Ro52 mRNA and protein expression. Increased Ro52 expression was also reported in tissues from patients with nonphotoaccentuated inflammatory skin disease, suggesting a broad role for Ro52 in cutaneous inflammation. Through the following questions, we examine this paper in greater detail. For brief answers, please refer to http://network.nature.com/group/jidclub. 1.What is Ro52?2.Describe how Ro52 monoclonal antibodies were developed and chosen for this study.3.Describe the patterns of Ro52 staining.4.What are the implications of this study’s findings?5.What were the limitations of this study? August 2009 Journal Club: Defining Ro Positivity -- Answers Download .pdf (.14 MB) Help with pdf files Supplementary MaterialAugust 2009 Journal Club: Defining Ro Positivity -- Answers" @default.
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• W2021990228 title "Defining Ro Positivity" @default.
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• W2021990228 doi "https://doi.org/10.1038/jid.2009.191" @default.
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