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- W2022020007 abstract "To transit between hosts, intracellular Legionella pneumophila transform into a motile, infectious, transmissive state. Here we exploit the pathogen's life cycle to examine how guanosine tetraphosphate (ppGpp) and DksA cooperate to govern bacterial differentiation. Transcriptional profiling revealed that during transmission alarmone accumulation increases the mRNA for flagellar and Type IV-secretion components, secreted host effectors and regulators, and decreases transcripts for translation, membrane modification and ATP synthesis machinery. DksA is critical for differentiation, since mutants are defective for stationary phase survival, flagellar gene activation, lysosome avoidance and macrophage cytotoxicity. The roles of ppGpp and DksA depend on the context. For macrophage transmission, ppGpp is essential, whereas DksA is dispensable, indicating that ppGpp can act autonomously. In broth, DksA promotes differentiation when ppGpp levels increase, or during fatty acid stress, as judged by flaA expression and evasion of degradation by macrophages. For flagella morphogenesis, DksA is required for basal fliA (sigma(28)) promoter activity. When alarmone levels increase, DksA cooperates with ppGpp to generate a pulse of Class II rod RNA or to amplify the Class III sigma factor and Class IV flagellin RNAs. Thus, DksA responds to the level of ppGpp and other stress signals to co-ordinate L. pneumophila differentiation." @default.
- W2022020007 created "2016-06-24" @default.
- W2022020007 creator A5031124304 @default.
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- W2022020007 creator A5055887257 @default.
- W2022020007 creator A5087862105 @default.
- W2022020007 date "2010-04-01" @default.
- W2022020007 modified "2023-10-16" @default.
- W2022020007 title "Distinct roles of ppGpp and DksA in<i>Legionella pneumophila</i>differentiation" @default.
- W2022020007 cites W1488674567 @default.
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- W2022020007 cites W1573477696 @default.
- W2022020007 cites W1848806194 @default.
- W2022020007 cites W1947628375 @default.
- W2022020007 cites W1967454460 @default.
- W2022020007 cites W1978581464 @default.
- W2022020007 cites W1980131133 @default.
- W2022020007 cites W1997878337 @default.
- W2022020007 cites W1998031206 @default.
- W2022020007 cites W1999355125 @default.
- W2022020007 cites W2000169636 @default.
- W2022020007 cites W2004393319 @default.
- W2022020007 cites W2007412334 @default.
- W2022020007 cites W2008034134 @default.
- W2022020007 cites W2008186432 @default.
- W2022020007 cites W2011958733 @default.
- W2022020007 cites W2015699985 @default.
- W2022020007 cites W2016814816 @default.
- W2022020007 cites W2019176322 @default.
- W2022020007 cites W2020212299 @default.
- W2022020007 cites W2023359102 @default.
- W2022020007 cites W2028812045 @default.
- W2022020007 cites W2036077981 @default.
- W2022020007 cites W2036631152 @default.
- W2022020007 cites W2036758334 @default.
- W2022020007 cites W2039160511 @default.
- W2022020007 cites W2039406539 @default.
- W2022020007 cites W2045789066 @default.
- W2022020007 cites W2046599852 @default.
- W2022020007 cites W2066210800 @default.
- W2022020007 cites W2069759547 @default.
- W2022020007 cites W2081283268 @default.
- W2022020007 cites W2081535038 @default.
- W2022020007 cites W2081856865 @default.
- W2022020007 cites W2085528794 @default.
- W2022020007 cites W2087664087 @default.
- W2022020007 cites W2095251670 @default.
- W2022020007 cites W2097101221 @default.
- W2022020007 cites W2097551453 @default.
- W2022020007 cites W2101058428 @default.
- W2022020007 cites W2102633503 @default.
- W2022020007 cites W2105028012 @default.
- W2022020007 cites W2105135172 @default.
- W2022020007 cites W2110276933 @default.
- W2022020007 cites W2111310696 @default.
- W2022020007 cites W2112864683 @default.
- W2022020007 cites W2116137883 @default.
- W2022020007 cites W2116765105 @default.
- W2022020007 cites W2117875503 @default.
- W2022020007 cites W2121915577 @default.
- W2022020007 cites W2122498796 @default.
- W2022020007 cites W2123076427 @default.
- W2022020007 cites W2125983377 @default.
- W2022020007 cites W2128954452 @default.
- W2022020007 cites W2128974582 @default.
- W2022020007 cites W2136670217 @default.
- W2022020007 cites W2138611595 @default.
- W2022020007 cites W2139543775 @default.
- W2022020007 cites W2147498477 @default.
- W2022020007 cites W2156241274 @default.
- W2022020007 cites W2157685329 @default.
- W2022020007 cites W2158435436 @default.
- W2022020007 cites W2158973900 @default.
- W2022020007 cites W2161320421 @default.
- W2022020007 cites W2161756577 @default.
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- W2022020007 doi "https://doi.org/10.1111/j.1365-2958.2010.07094.x" @default.
- W2022020007 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/2908999" @default.
- W2022020007 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/20199605" @default.
- W2022020007 hasPublicationYear "2010" @default.
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