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• W2022022102 startingPage "1067" @default.
• W2022022102 abstract "Many fluorescent reagents with a benzofurazan (2,1,3-benzoxadiazole) skeleton have been developed and widely used in bio-analyses. In this study, we try to elucidate the fluorescence on-off switching mechanism of three fluorogenic reagents and their derivatives. Ten 4,7-disubstituted benzofurazans were used for this purpose and the measurements of their fluorescence, phosphorescence, photolysis, and time-resolved thermal lensing signal in acetonitrile were obtained in order to understand the relaxation processes of these compounds. These results indicate that the competition of fluorescence with a fast intersystem crossing or fast photoreaction plays a key role in the fluorescence on-off switching. Semi-empirical molecular orbital calculations show that the existence of the triplet n pi* state is responsible for the fast intersystem crossing while the proximity of the reactive second single pi pi* state to the first singlet pi pi* state contributes to the fast photoreaction in the excited states." @default.
• W2022022102 created "2016-06-24" @default.
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• W2022022102 date "2003-02-17" @default.
• W2022022102 modified "2023-10-01" @default.
• W2022022102 title "Fluorescence on–off switching mechanism of benzofurazans" @default.
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• W2022022102 doi "https://doi.org/10.1039/b212575g" @default.
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