Matches in SemOpenAlex for { <https://semopenalex.org/work/W2022030421> ?p ?o ?g. }
Showing items 1 to 95 of
95
with 100 items per page.
- W2022030421 endingPage "404" @default.
- W2022030421 startingPage "397" @default.
- W2022030421 abstract "NAD is normally regarded as a redox molecule or as the substrate for ADP-ribosylation reactions. In this study, we describe the rapid metabolism of NAD by Percoll-gradient-purified lettuce chloroplasts and show that the adenine moiety can be incorporated into RNA in a dark-activated reaction that senses the redox state of the cytochrome b6f complex. Isolated chloroplasts rapidly metabolised radiolabelled NAD+ to 5′-AMP (within seconds) and adenosine during a 60-min incubation in vitro; the products were analysed by high-performance liquid chromatography. No radiolabelled ADP-ribose was detected. Radioactivity was incorporated into trichloroacetic-acid-insoluble material during this period, with approximately 2–4-fold more incorporation occurring in the dark. Most of this radiolabel was rendered acid-soluble by dilute alkaline digestion at 37°C, yielding an approximately equal mixture of 2′-AMP and 3′-AMP, and by RNase digestion, identifying the acid-insoluble radioactive material as RNA. Protein-bound ADP-ribose would have yielded 5′-AMP and/ or oligomeric/polymeric ADP-ribose after alkali digestion. The utilisation of NAD metabolites for RNA synthesis was restricted to the thylakoid compartment of the chloroplast. The use of a variety of electron-transport inhibitors such as 2,5-dibromo-3-methyl-6-isopropyl-p-benzoquinone, bromanil (tetrabromo-1,4-benzoquinone), electron donors (dithiothreitol), electron acceptors (ferricyanide) and an uncoupler showed that the incorporation of radiolabel from NAD into acid-insoluble material was favoured when the cytochrome b6f complex was in the oxidised state (as pertaining to incubations in the dark)." @default.
- W2022030421 created "2016-06-24" @default.
- W2022030421 creator A5007456436 @default.
- W2022030421 creator A5019310178 @default.
- W2022030421 creator A5004825819 @default.
- W2022030421 creator A5015612492 @default.
- W2022030421 date "1993-12-01" @default.
- W2022030421 modified "2023-09-26" @default.
- W2022030421 title "NAD turnover and utilisation of metabolites for RNA synthesis in a reaction sensing the redox state of the cytochrome b6f complex in isolated chloroplasts" @default.
- W2022030421 cites W1186306548 @default.
- W2022030421 cites W1434809468 @default.
- W2022030421 cites W1482146624 @default.
- W2022030421 cites W1595653370 @default.
- W2022030421 cites W161771070 @default.
- W2022030421 cites W1965629862 @default.
- W2022030421 cites W1981718931 @default.
- W2022030421 cites W198719626 @default.
- W2022030421 cites W2006290933 @default.
- W2022030421 cites W2012103229 @default.
- W2022030421 cites W2012859479 @default.
- W2022030421 cites W2013423213 @default.
- W2022030421 cites W2026329804 @default.
- W2022030421 cites W2032644226 @default.
- W2022030421 cites W2034793491 @default.
- W2022030421 cites W2038948001 @default.
- W2022030421 cites W2068356785 @default.
- W2022030421 cites W2086785571 @default.
- W2022030421 cites W2107610880 @default.
- W2022030421 cites W2134812217 @default.
- W2022030421 cites W2283006446 @default.
- W2022030421 cites W2345134020 @default.
- W2022030421 cites W4252728709 @default.
- W2022030421 cites W600855677 @default.
- W2022030421 cites W2050196180 @default.
- W2022030421 cites W2584383172 @default.
- W2022030421 doi "https://doi.org/10.1111/j.1432-1033.1993.tb18389.x" @default.
- W2022030421 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/7505745" @default.
- W2022030421 hasPublicationYear "1993" @default.
- W2022030421 type Work @default.
- W2022030421 sameAs 2022030421 @default.
- W2022030421 citedByCount "34" @default.
- W2022030421 countsByYear W20220304212015 @default.
- W2022030421 countsByYear W20220304212018 @default.
- W2022030421 crossrefType "journal-article" @default.
- W2022030421 hasAuthorship W2022030421A5004825819 @default.
- W2022030421 hasAuthorship W2022030421A5007456436 @default.
- W2022030421 hasAuthorship W2022030421A5015612492 @default.
- W2022030421 hasAuthorship W2022030421A5019310178 @default.
- W2022030421 hasBestOaLocation W20220304211 @default.
- W2022030421 hasConcept C104317684 @default.
- W2022030421 hasConcept C161790260 @default.
- W2022030421 hasConcept C178790620 @default.
- W2022030421 hasConcept C181199279 @default.
- W2022030421 hasConcept C185592680 @default.
- W2022030421 hasConcept C2776651935 @default.
- W2022030421 hasConcept C2777411777 @default.
- W2022030421 hasConcept C2780768313 @default.
- W2022030421 hasConcept C55493867 @default.
- W2022030421 hasConcept C55904794 @default.
- W2022030421 hasConcept C69305403 @default.
- W2022030421 hasConcept C75520062 @default.
- W2022030421 hasConceptScore W2022030421C104317684 @default.
- W2022030421 hasConceptScore W2022030421C161790260 @default.
- W2022030421 hasConceptScore W2022030421C178790620 @default.
- W2022030421 hasConceptScore W2022030421C181199279 @default.
- W2022030421 hasConceptScore W2022030421C185592680 @default.
- W2022030421 hasConceptScore W2022030421C2776651935 @default.
- W2022030421 hasConceptScore W2022030421C2777411777 @default.
- W2022030421 hasConceptScore W2022030421C2780768313 @default.
- W2022030421 hasConceptScore W2022030421C55493867 @default.
- W2022030421 hasConceptScore W2022030421C55904794 @default.
- W2022030421 hasConceptScore W2022030421C69305403 @default.
- W2022030421 hasConceptScore W2022030421C75520062 @default.
- W2022030421 hasIssue "2" @default.
- W2022030421 hasLocation W20220304211 @default.
- W2022030421 hasLocation W20220304212 @default.
- W2022030421 hasOpenAccess W2022030421 @default.
- W2022030421 hasPrimaryLocation W20220304211 @default.
- W2022030421 hasRelatedWork W1978962336 @default.
- W2022030421 hasRelatedWork W2021099451 @default.
- W2022030421 hasRelatedWork W2053988811 @default.
- W2022030421 hasRelatedWork W2083666555 @default.
- W2022030421 hasRelatedWork W2431308298 @default.
- W2022030421 hasRelatedWork W2952478468 @default.
- W2022030421 hasRelatedWork W3206551310 @default.
- W2022030421 hasRelatedWork W348967882 @default.
- W2022030421 hasRelatedWork W2016169741 @default.
- W2022030421 hasRelatedWork W2465427213 @default.
- W2022030421 hasVolume "218" @default.
- W2022030421 isParatext "false" @default.
- W2022030421 isRetracted "false" @default.
- W2022030421 magId "2022030421" @default.
- W2022030421 workType "article" @default.