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• W2022032586 abstract "Abstract In the human eye, domination of the electroretinogram (ERG) by the b −wave and other postreceptor components ordinarily obscures all but the first few milliseconds of the rod photoreceptor response to a stimulating flash. However, recovery of the rod response after a bright test flash can be analyzed using a paired-flash paradigm in which the test flash, presented at time zero, is followed at time t by a bright probe flash that rapidly saturates the rods (Birch et al., 1995). In ERG experiments on normal subjects, the hypothesis that a similar method can be used to obtain the full time course of the rod response to test flashes of subsaturating intensity was tested. Rod-only responses to probe flashes presented at varying times t after the test flash were used to derive a family of amplitudes A ( t ) that represented the putative rod response to the test flash. These rod-only responses to the probe flash were obtained by computational subtraction of the cone-mediated component of each probe flash response. With relatively weak test flashes (11–15 scot-td-s), the time course of the rod response to the test flash derived in this manner was consistent with a four-stage impulse response function of time-to-peak ≃170 ms. A (170), the amplitude of the derived response at 170 ms, increased with test flash intensity ( I test ) to a maximum value A mo and exhibited a dependence on I test given approximately by the relation, A (170)/ A mo = 1 - exp(-k I test ), where k = 0.092 (scot-td-s) −1 . In steady background light, the falling (i.e. recovery) phase of the derived response began earlier, and the sensitivity parameter k was reduced several-fold from its dark-adapted value. As the sensitivity, kinetics, and light-adaptation properties of the derived response correspond closely with those of photocurrent flash responses previously obtained from isolated rods in vitro , it was concluded that the response derived here from the human ERG approximates the course of the massed in vivo rod response to a test flash." @default.
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• W2022032586 date "1997-01-01" @default.
• W2022032586 modified "2023-10-13" @default.
• W2022032586 title "Photoresponses of human rods <i>in vivo</i> derived from paired-flash electroretinograms" @default.
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• W2022032586 doi "https://doi.org/10.1017/s0952523800008774" @default.
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