Matches in SemOpenAlex for { <https://semopenalex.org/work/W2022040726> ?p ?o ?g. }
Showing items 1 to 78 of
78
with 100 items per page.
- W2022040726 endingPage "334" @default.
- W2022040726 startingPage "333" @default.
- W2022040726 abstract "In addition to the somatodendritic region, myenteric motoneuron terminals are endowed with nicotinic autoreceptors. We aimed at investigating the effect of nicotinic receptor (nAChR) activation on [3H]-acetylcholine ([3H]-ACh) release from longitudinal muscle-myenteric plexus of the rat ileum and to evaluate whether this could be modulated by adenosine, an endogenous neuromodulator typically operating changes in intracellular cyclic AMP. The nAChR agonist, 1,1-dimethyl-4-phenylpiperazinium (DMPP, 1–30 μM, 3 min) increased [3H]-ACh release in a concentration-dependent manner. DMPP (30 μM)-induced [3H]-ACh outflow was attenuated by hexamethonium (0.1–1 mM), tubocurarine (1–5 μM), or by removing external Ca2+ (plus EGTA, 1 mM). In contrast to veratridine (0.2–10 μM)-induced [3H]-ACh release, the DMPP (30 μM)-induced outflow was resistant to tetrodotoxin (1 μM) and cadmium (0.5 mM). Pretreatment with adenosine deaminase (0.5 U/mL) or with the adenosine A2A-receptor antagonist, ZM 241385 (50 nM), enhanced nAChR-induced transmitter release. Activation of A2A receptors with CGS 21680C (3 nM) reduced the DMPP-induced release of [3H]-ACh. CGS 21680C (3 nM) inhibition was prevented by MDL 12,330A (10 μM, an adenylate cyclase inhibitor) and by H-89 (10 μM, an inhibitor of protein kinase A), but was potentiated by rolipram (300 μM, a phosphodiesterase inhibitor). DMPP-induced transmitter release was decreased by 8-bromo-cyclic AMP (1 mM, a protein kinase A activator), rolipram (300 μM), and forskolin (3 μM, an activator of adenylate cyclase). Both MDL 12,330A (10 μM) and H-89 (10 μM) facilitated DMPP-induced release of [3H]-ACh.The results indicate that nAChR-induced [3H]-ACh release is triggered by the influx of Ca2+, independent of voltage-sensitive calcium channels, presumably directly through nAChRs located on myenteric axon terminals. It was also shown that endogenous adenosine, activating A2A receptors coupled to the adenylate cyclase/cyclic AMP transducing system, is tonically downregulating this nAChR-mediated control of [3H]-ACh release." @default.
- W2022040726 created "2016-06-24" @default.
- W2022040726 creator A5052221889 @default.
- W2022040726 date "1982-04-01" @default.
- W2022040726 modified "2023-10-15" @default.
- W2022040726 title "Release of ATP from isolated myenteric varicosities by nicotinic agonists" @default.
- W2022040726 cites W1802887711 @default.
- W2022040726 cites W2086701079 @default.
- W2022040726 cites W2152426612 @default.
- W2022040726 doi "https://doi.org/10.1016/0014-2999(82)90643-4" @default.
- W2022040726 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/6124434" @default.
- W2022040726 hasPublicationYear "1982" @default.
- W2022040726 type Work @default.
- W2022040726 sameAs 2022040726 @default.
- W2022040726 citedByCount "25" @default.
- W2022040726 countsByYear W20220407262012 @default.
- W2022040726 crossrefType "journal-article" @default.
- W2022040726 hasAuthorship W2022040726A5052221889 @default.
- W2022040726 hasConcept C126322002 @default.
- W2022040726 hasConcept C134018914 @default.
- W2022040726 hasConcept C170493617 @default.
- W2022040726 hasConcept C178790620 @default.
- W2022040726 hasConcept C181199279 @default.
- W2022040726 hasConcept C185592680 @default.
- W2022040726 hasConcept C2775893369 @default.
- W2022040726 hasConcept C2775910092 @default.
- W2022040726 hasConcept C2776958096 @default.
- W2022040726 hasConcept C2776986425 @default.
- W2022040726 hasConcept C2776991684 @default.
- W2022040726 hasConcept C2778938600 @default.
- W2022040726 hasConcept C2779276759 @default.
- W2022040726 hasConcept C50952357 @default.
- W2022040726 hasConcept C537181965 @default.
- W2022040726 hasConcept C55493867 @default.
- W2022040726 hasConcept C62826618 @default.
- W2022040726 hasConcept C71924100 @default.
- W2022040726 hasConcept C86803240 @default.
- W2022040726 hasConceptScore W2022040726C126322002 @default.
- W2022040726 hasConceptScore W2022040726C134018914 @default.
- W2022040726 hasConceptScore W2022040726C170493617 @default.
- W2022040726 hasConceptScore W2022040726C178790620 @default.
- W2022040726 hasConceptScore W2022040726C181199279 @default.
- W2022040726 hasConceptScore W2022040726C185592680 @default.
- W2022040726 hasConceptScore W2022040726C2775893369 @default.
- W2022040726 hasConceptScore W2022040726C2775910092 @default.
- W2022040726 hasConceptScore W2022040726C2776958096 @default.
- W2022040726 hasConceptScore W2022040726C2776986425 @default.
- W2022040726 hasConceptScore W2022040726C2776991684 @default.
- W2022040726 hasConceptScore W2022040726C2778938600 @default.
- W2022040726 hasConceptScore W2022040726C2779276759 @default.
- W2022040726 hasConceptScore W2022040726C50952357 @default.
- W2022040726 hasConceptScore W2022040726C537181965 @default.
- W2022040726 hasConceptScore W2022040726C55493867 @default.
- W2022040726 hasConceptScore W2022040726C62826618 @default.
- W2022040726 hasConceptScore W2022040726C71924100 @default.
- W2022040726 hasConceptScore W2022040726C86803240 @default.
- W2022040726 hasIssue "3-4" @default.
- W2022040726 hasLocation W20220407261 @default.
- W2022040726 hasLocation W20220407262 @default.
- W2022040726 hasOpenAccess W2022040726 @default.
- W2022040726 hasPrimaryLocation W20220407261 @default.
- W2022040726 hasRelatedWork W1980580132 @default.
- W2022040726 hasRelatedWork W1995778722 @default.
- W2022040726 hasRelatedWork W2000265387 @default.
- W2022040726 hasRelatedWork W2004450714 @default.
- W2022040726 hasRelatedWork W2005975503 @default.
- W2022040726 hasRelatedWork W2015572523 @default.
- W2022040726 hasRelatedWork W2046921980 @default.
- W2022040726 hasRelatedWork W2068180445 @default.
- W2022040726 hasRelatedWork W2071346914 @default.
- W2022040726 hasRelatedWork W2145857953 @default.
- W2022040726 hasVolume "79" @default.
- W2022040726 isParatext "false" @default.
- W2022040726 isRetracted "false" @default.
- W2022040726 magId "2022040726" @default.
- W2022040726 workType "article" @default.