Matches in SemOpenAlex for { <https://semopenalex.org/work/W2022042465> ?p ?o ?g. }
- W2022042465 endingPage "4465" @default.
- W2022042465 startingPage "4453" @default.
- W2022042465 abstract "Sequence diversity within a family of functional enzymes provides a platform for elucidating structure-function relationships and for protein engineering to improve properties important for applications. Access to nature's vast sequence diversity is often limited by the fact that only a few enzymes have been characterized in a given family. Here, we recombined the catalytic domains of three glycoside hydrolase family 48 bacterial cellulases (Cel48; EC 3.2.1.176) - Clostridium cellulolyticum CelF, Clostridium stercorarium CelY, and Clostridium thermocellum CelS - to create a diverse library of Cel48 enzymes with an average of 106 mutations from the closest native enzyme. Within this set, we found large variations in properties such as the functional temperature range, stability, and specific activity on crystalline cellulose. We showed that functional status and stability were predictable from simple linear models of the sequence-property data: recombined protein fragments contributed additively to these properties in a given chimera. Using this, we correctly predicted sequences that were as stable as any of the native Cel48 enzymes described to date. The characterization of 60 active Cel48 chimeras expands the number of characterized Cel48 enzymes from 13 to 73. Our work illustrates the role that structure-guided recombination can play in helping to identify sequence-function relationships within a family of enzymes by supplementing natural diversity with synthetic diversity." @default.
- W2022042465 created "2016-06-24" @default.
- W2022042465 creator A5013916255 @default.
- W2022042465 creator A5018035456 @default.
- W2022042465 creator A5031109529 @default.
- W2022042465 creator A5053465701 @default.
- W2022042465 creator A5063961340 @default.
- W2022042465 creator A5074944933 @default.
- W2022042465 creator A5081226757 @default.
- W2022042465 date "2012-11-09" @default.
- W2022042465 modified "2023-09-25" @default.
- W2022042465 title "A diverse set of family 48 bacterial glycoside hydrolase cellulases created by structure-guided recombination" @default.
- W2022042465 cites W1543739240 @default.
- W2022042465 cites W1561750927 @default.
- W2022042465 cites W1568630772 @default.
- W2022042465 cites W1793247465 @default.
- W2022042465 cites W1969439217 @default.
- W2022042465 cites W1988966516 @default.
- W2022042465 cites W1995574044 @default.
- W2022042465 cites W2001002058 @default.
- W2022042465 cites W2001952758 @default.
- W2022042465 cites W2006223695 @default.
- W2022042465 cites W2008450196 @default.
- W2022042465 cites W2017474351 @default.
- W2022042465 cites W2018785303 @default.
- W2022042465 cites W2019200192 @default.
- W2022042465 cites W2032921415 @default.
- W2022042465 cites W2041251751 @default.
- W2022042465 cites W2056636001 @default.
- W2022042465 cites W2058791440 @default.
- W2022042465 cites W2098787289 @default.
- W2022042465 cites W2098839738 @default.
- W2022042465 cites W2099560117 @default.
- W2022042465 cites W2103427109 @default.
- W2022042465 cites W2108929776 @default.
- W2022042465 cites W2134359468 @default.
- W2022042465 cites W2135211698 @default.
- W2022042465 cites W2135228920 @default.
- W2022042465 cites W2141346454 @default.
- W2022042465 cites W2149595091 @default.
- W2022042465 cites W2150053831 @default.
- W2022042465 cites W2159090193 @default.
- W2022042465 cites W2169346156 @default.
- W2022042465 cites W2487574160 @default.
- W2022042465 cites W4294541781 @default.
- W2022042465 doi "https://doi.org/10.1111/febs.12032" @default.
- W2022042465 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/23075376" @default.
- W2022042465 hasPublicationYear "2012" @default.
- W2022042465 type Work @default.
- W2022042465 sameAs 2022042465 @default.
- W2022042465 citedByCount "42" @default.
- W2022042465 countsByYear W20220424652012 @default.
- W2022042465 countsByYear W20220424652013 @default.
- W2022042465 countsByYear W20220424652014 @default.
- W2022042465 countsByYear W20220424652015 @default.
- W2022042465 countsByYear W20220424652016 @default.
- W2022042465 countsByYear W20220424652017 @default.
- W2022042465 countsByYear W20220424652018 @default.
- W2022042465 countsByYear W20220424652019 @default.
- W2022042465 countsByYear W20220424652020 @default.
- W2022042465 countsByYear W20220424652022 @default.
- W2022042465 countsByYear W20220424652023 @default.
- W2022042465 crossrefType "journal-article" @default.
- W2022042465 hasAuthorship W2022042465A5013916255 @default.
- W2022042465 hasAuthorship W2022042465A5018035456 @default.
- W2022042465 hasAuthorship W2022042465A5031109529 @default.
- W2022042465 hasAuthorship W2022042465A5053465701 @default.
- W2022042465 hasAuthorship W2022042465A5063961340 @default.
- W2022042465 hasAuthorship W2022042465A5074944933 @default.
- W2022042465 hasAuthorship W2022042465A5081226757 @default.
- W2022042465 hasBestOaLocation W20220424651 @default.
- W2022042465 hasConcept C104317684 @default.
- W2022042465 hasConcept C141603559 @default.
- W2022042465 hasConcept C147816474 @default.
- W2022042465 hasConcept C15151743 @default.
- W2022042465 hasConcept C181199279 @default.
- W2022042465 hasConcept C196032511 @default.
- W2022042465 hasConcept C2776061948 @default.
- W2022042465 hasConcept C2780120296 @default.
- W2022042465 hasConcept C54355233 @default.
- W2022042465 hasConcept C55493867 @default.
- W2022042465 hasConcept C70721500 @default.
- W2022042465 hasConcept C86803240 @default.
- W2022042465 hasConceptScore W2022042465C104317684 @default.
- W2022042465 hasConceptScore W2022042465C141603559 @default.
- W2022042465 hasConceptScore W2022042465C147816474 @default.
- W2022042465 hasConceptScore W2022042465C15151743 @default.
- W2022042465 hasConceptScore W2022042465C181199279 @default.
- W2022042465 hasConceptScore W2022042465C196032511 @default.
- W2022042465 hasConceptScore W2022042465C2776061948 @default.
- W2022042465 hasConceptScore W2022042465C2780120296 @default.
- W2022042465 hasConceptScore W2022042465C54355233 @default.
- W2022042465 hasConceptScore W2022042465C55493867 @default.
- W2022042465 hasConceptScore W2022042465C70721500 @default.
- W2022042465 hasConceptScore W2022042465C86803240 @default.
- W2022042465 hasIssue "24" @default.
- W2022042465 hasLocation W20220424651 @default.
- W2022042465 hasLocation W20220424652 @default.