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• W2022044712 abstract "The goal of the present study was to test the hypothesis that a second, more distal, promoter exists upstream from the rat prolactin (PRL) gene (r Prl ) that is homologous to that identified upstream from the human prolactin gene (h Prl ). The nucleotide sequence of the rat genome extending from 7.4 to 2.5 kb upstream from the proximal r Prl promoter was determined, revealing significant sequence homology to the h Prl distal promoter and its 5′-flanking domain. Using reverse transcription-polymerase chain reaction , novel r Prl transcripts that originate upstream from the proximal r Prl promoter were detected in rat uterus, spleen, pons medulla, anterior pituitary and the GH4C1 pituitary tumor cell line. Further characterization of these novel 5′-extended r Prl transcripts from GH4C1 cells indicated that they were full length, polyadenylated and properly spliced. However, data from primer extension (5′-RACE) experiments strongly suggested that the 5′-extended r Prl transcripts originate, not at the distal promoter-like motif, but at scattered sites located 60–153 bp upstream from the proximal promoter. Therefore, it appears improbable that the rat sequences homologous to the h Prl distal promoter comprise a functional promoter." @default.
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• W2022044712 date "1998-11-01" @default.
• W2022044712 modified "2023-09-24" @default.
• W2022044712 title "Identification in the rat prolactin gene of sequences homologous to the distal promoter of the human prolactin gene" @default.
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• W2022044712 doi "https://doi.org/10.1016/s0167-4781(98)00172-9" @default.
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