Matches in SemOpenAlex for { <https://semopenalex.org/work/W2022046477> ?p ?o ?g. }
- W2022046477 endingPage "579" @default.
- W2022046477 startingPage "576" @default.
- W2022046477 abstract "Kinetochores are macromolecular machines that couple chromosomes to dynamic microtubule tips during cell division, thereby generating force to segregate the chromosomes. Accurate segregation depends on selective stabilization of correct 'bi-oriented' kinetochore-microtubule attachments, which come under tension as the result of opposing forces exerted by microtubules. Tension is thought to stabilize these bi-oriented attachments indirectly, by suppressing the destabilizing activity of a kinase, Aurora B. However, a complete mechanistic understanding of the role of tension requires reconstitution of kinetochore-microtubule attachments for biochemical and biophysical analyses in vitro. Here we show that native kinetochore particles retaining the majority of kinetochore proteins can be purified from budding yeast and used to reconstitute dynamic microtubule attachments. Individual kinetochore particles maintain load-bearing associations with assembling and disassembling ends of single microtubules for >30 min, providing a close match to the persistent coupling seen in vivo between budding yeast kinetochores and single microtubules. Moreover, tension increases the lifetimes of the reconstituted attachments directly, through a catch bond-like mechanism that does not require Aurora B. On the basis of these findings, we propose that tension selectively stabilizes proper kinetochore-microtubule attachments in vivo through a combination of direct mechanical stabilization and tension-dependent phosphoregulation." @default.
- W2022046477 created "2016-06-24" @default.
- W2022046477 creator A5005300159 @default.
- W2022046477 creator A5010162864 @default.
- W2022046477 creator A5013591755 @default.
- W2022046477 creator A5020372858 @default.
- W2022046477 creator A5031038205 @default.
- W2022046477 creator A5038047977 @default.
- W2022046477 creator A5060873474 @default.
- W2022046477 creator A5074569982 @default.
- W2022046477 creator A5084200031 @default.
- W2022046477 creator A5087456716 @default.
- W2022046477 date "2010-11-01" @default.
- W2022046477 modified "2023-10-16" @default.
- W2022046477 title "Tension directly stabilizes reconstituted kinetochore-microtubule attachments" @default.
- W2022046477 cites W1520255714 @default.
- W2022046477 cites W1523096427 @default.
- W2022046477 cites W1561777275 @default.
- W2022046477 cites W1965614813 @default.
- W2022046477 cites W1966072209 @default.
- W2022046477 cites W1967696278 @default.
- W2022046477 cites W1968407352 @default.
- W2022046477 cites W1969299223 @default.
- W2022046477 cites W1980166711 @default.
- W2022046477 cites W1997206295 @default.
- W2022046477 cites W1997535646 @default.
- W2022046477 cites W2012454516 @default.
- W2022046477 cites W2031284400 @default.
- W2022046477 cites W2037170319 @default.
- W2022046477 cites W2044730950 @default.
- W2022046477 cites W2046134480 @default.
- W2022046477 cites W2052192720 @default.
- W2022046477 cites W2084223205 @default.
- W2022046477 cites W2088118120 @default.
- W2022046477 cites W2090834457 @default.
- W2022046477 cites W2104418223 @default.
- W2022046477 cites W2115364257 @default.
- W2022046477 cites W2115960102 @default.
- W2022046477 cites W2119108223 @default.
- W2022046477 cites W2120125134 @default.
- W2022046477 cites W2120623923 @default.
- W2022046477 cites W2124653599 @default.
- W2022046477 cites W2125483939 @default.
- W2022046477 cites W2126448233 @default.
- W2022046477 cites W2132547886 @default.
- W2022046477 cites W2138233324 @default.
- W2022046477 cites W2155708268 @default.
- W2022046477 cites W2157980476 @default.
- W2022046477 cites W2158169016 @default.
- W2022046477 cites W2160648230 @default.
- W2022046477 cites W2161666027 @default.
- W2022046477 cites W2166102124 @default.
- W2022046477 cites W2169333620 @default.
- W2022046477 doi "https://doi.org/10.1038/nature09594" @default.
- W2022046477 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3108429" @default.
- W2022046477 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/21107429" @default.
- W2022046477 hasPublicationYear "2010" @default.
- W2022046477 type Work @default.
- W2022046477 sameAs 2022046477 @default.
- W2022046477 citedByCount "402" @default.
- W2022046477 countsByYear W20220464772012 @default.
- W2022046477 countsByYear W20220464772013 @default.
- W2022046477 countsByYear W20220464772014 @default.
- W2022046477 countsByYear W20220464772015 @default.
- W2022046477 countsByYear W20220464772016 @default.
- W2022046477 countsByYear W20220464772017 @default.
- W2022046477 countsByYear W20220464772018 @default.
- W2022046477 countsByYear W20220464772019 @default.
- W2022046477 countsByYear W20220464772020 @default.
- W2022046477 countsByYear W20220464772021 @default.
- W2022046477 countsByYear W20220464772022 @default.
- W2022046477 countsByYear W20220464772023 @default.
- W2022046477 crossrefType "journal-article" @default.
- W2022046477 hasAuthorship W2022046477A5005300159 @default.
- W2022046477 hasAuthorship W2022046477A5010162864 @default.
- W2022046477 hasAuthorship W2022046477A5013591755 @default.
- W2022046477 hasAuthorship W2022046477A5020372858 @default.
- W2022046477 hasAuthorship W2022046477A5031038205 @default.
- W2022046477 hasAuthorship W2022046477A5038047977 @default.
- W2022046477 hasAuthorship W2022046477A5060873474 @default.
- W2022046477 hasAuthorship W2022046477A5074569982 @default.
- W2022046477 hasAuthorship W2022046477A5084200031 @default.
- W2022046477 hasAuthorship W2022046477A5087456716 @default.
- W2022046477 hasBestOaLocation W20220464772 @default.
- W2022046477 hasConcept C104317684 @default.
- W2022046477 hasConcept C12554922 @default.
- W2022046477 hasConcept C141937037 @default.
- W2022046477 hasConcept C1491633281 @default.
- W2022046477 hasConcept C185592680 @default.
- W2022046477 hasConcept C192838845 @default.
- W2022046477 hasConcept C20418707 @default.
- W2022046477 hasConcept C2778354886 @default.
- W2022046477 hasConcept C30481170 @default.
- W2022046477 hasConcept C47432576 @default.
- W2022046477 hasConcept C54355233 @default.
- W2022046477 hasConcept C85813293 @default.
- W2022046477 hasConcept C86803240 @default.
- W2022046477 hasConcept C95444343 @default.