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- W2022046632 abstract "Whilst many active and passive immunization approaches have been developed with limited success, for Alzheimer's disease (AD), immunomodulation has been less studied for prion diseases which involve the conversion of cellular PrP C into a toxic and infectious prion PrP Res. Recent studies by our group has demonstrated that active mucosal immunization is a feasible therapeutic approach to prevent transmission and progression in mouse and deer. In the course of producing conformational monoclonal antibodies (mAbs) with different binding capacities for the Aβ and hyperphosphorylated Tau pathological conformers; we developed a few clones that showed distinct activity towards PrP Res using ELISA. These mAbs were developed by selecting clones that recognize β-sheet oligomeric structures. Such mAbs might be potential tools for detecting, diagnosing and treating prionoses. From a few mAbs that by Western blot showed specific binding to monomeric and multimeric prions we selected one (TAP1) for further characterization. Monoclonal antibodies obtained from our conformational immunization of BALBc mice using a polymerized 13mer Bri peptide (pBri) were tested for reactivity towards different forms of PrP in ELISA. Selected TAP1 was further characterized by Western blots, ELISAs, immunohistochemistry using human GSS tissue, and potential inhibition of in vitro 22L prion infection of N2a cells. TAP1 reacted strongly in ELISA against different PrP Res preparations. By Western blot it detected oligomeric forms of prions after PK digestion, including samples from human CJD. Using immunofluorescence we detected specific immunolabeling of neurons from human GSS cases. TAP1 was also able to significantly reduce infection of N2a cells by 22L prions. We characterized a monoclonal antibody that not only detects AD related pathology but strongly reacted with different oligomeric forms of PrP Res. This type of mAb can be of use for the detection of different prion forms, contributing to the diagnosis, monitoring of disease progress and potential therapy for prion diseases." @default.
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- W2022046632 date "2014-07-01" @default.
- W2022046632 modified "2023-09-25" @default.
- W2022046632 title "P4-372: ANTI-CONFORMATIONAL MONOCLONAL ANTIBODY WITH SELECTIVE PREFERENCE FOR PRP RES STRAINS FROM DIFFERENT ANIMAL SPECIES AND HUMANS" @default.
- W2022046632 doi "https://doi.org/10.1016/j.jalz.2014.07.141" @default.
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