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• W2022047822 abstract "Paenibacillus polymyxa A-8, which secretes β-mannosidase, was isolated from the soil sample under a pine tree located in the Laoban mountain region of Sichuan, China. The β-mannosidase gene (MANB) was isolated from P. polymyxa A-8, using primers according to the complete genome. The MANB (2,550 bp) encoding 849 amino acid residues was expressed in Escherichia coli. The specific activities of β-mannosidase produced by P. polymyxa A-8 and E. coli pET30a-MANB were 12 nkat/mg and 635 nkat/mg respectively. SDS-PAGE analysis indicated that the molecular mass of the recombinant MANB was approximately 96 kDa. The recombinant MANB was active between pH 7.0-8.5 with the maximum activity at pH 7.0. It had good pH stability and adaptability. The MANB had the optimal temperature of 35°C and was relatively stable at 35-40°C. In addition, the MANB activity was enhanced by K+, Ca2+, Mn2+, and Mg2+ and inhibited by Zn2+, Cu2+, and Hg2+." @default.
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• W2022047822 date "2014-11-25" @default.
• W2022047822 modified "2023-10-01" @default.
• W2022047822 title "Expression of a β-Mannosidase from Paenibacillus polymyxa A-8 in Escherichia coli and Characterization of the Recombinant Enzyme" @default.
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• W2022047822 doi "https://doi.org/10.1371/journal.pone.0111622" @default.
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