FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2022048689> ?p ?o ?g. }

• W2022048689 endingPage "391" @default.
• W2022048689 startingPage "385" @default.
• W2022048689 abstract "M c a - G l y - A s p - A l a - G l u - T y r ( P O3H2 ) - A l a - A l a - L y s ( D N P ) - A r g-NH2, where Mca is (7-methoxycoumarin-4-yl)acetyl and DNP is 2,4-dinitrophenyl, was synthesized as a fluorogenic substrate for protein tyrosine phosphatases (PTPs). In the peptide, the fluorescent Mca group is quenched efficiently by the DNP group. Although the fluorescence intensity of the substrate was practically unchanged upon PTP-catalysed dephosphorylation, it increased approx. 120-fold upon subsequent treatment with chymotrypsin. Analysis by HPLC showed that chymotrypsin cleaved only the dephosphorylated substrate at the Tyr-Ala bond. Thus with the aid of chymotrypsin, dephosphorylation of the substrate can be measured fluorometrically. A strictly linear correlation was observed between PTP concentration and dephosphorylation rate. The fluorogenic substrate was dephosphorylated by some PTPs much more rapidly than the corresponding 32P-labelled substrate used for comparison, whereas alkaline phosphatase dephosphorylated the two substrates at similar rates. The fluorogenic substrate is therefore more specific for PTPs than the radiolabelled substrate. The assay with the fluorogenic substrate could be applied to the estimation of kinetc parameters and measurement of PTP activity in crude-enzyme preparations. The lower detection limit of our assay (1 μM substrate in 200 μl of reaction mixture) was estimated to be 0.2-0.4 pmol, whereas it was estimated to be about 1 pmol in the assay that used 32P-labelled peptide (specific radioactivity of approx. 1000 c.p.m./pmol). Our assay is simple, specific, highly sensitive and non-radioisotopic, and hence would contribute greatly to the development of PTP biology." @default.
• W2022048689 created "2016-06-24" @default.
• W2022048689 creator A5002044987 @default.
• W2022048689 creator A5025126230 @default.
• W2022048689 creator A5033027657 @default.
• W2022048689 creator A5071187146 @default.
• W2022048689 creator A5074937251 @default.
• W2022048689 date "1999-10-08" @default.
• W2022048689 modified "2023-10-10" @default.
• W2022048689 title "A phosphotyrosine-containing quenched fluorogenic peptide as a novel substrate for protein tyrosine phosphatases" @default.
• W2022048689 cites W1749352888 @default.
• W2022048689 cites W1975195021 @default.
• W2022048689 cites W1977158130 @default.
• W2022048689 cites W1979630058 @default.
• W2022048689 cites W1996522211 @default.
• W2022048689 cites W2003570140 @default.
• W2022048689 cites W2004356154 @default.
• W2022048689 cites W2010697345 @default.
• W2022048689 cites W2012514869 @default.
• W2022048689 cites W2026663568 @default.
• W2022048689 cites W2036754828 @default.
• W2022048689 cites W2050398273 @default.
• W2022048689 cites W2053320244 @default.
• W2022048689 cites W2062507551 @default.
• W2022048689 cites W2064248967 @default.
• W2022048689 cites W2073779740 @default.
• W2022048689 cites W2082796370 @default.
• W2022048689 cites W2089221489 @default.
• W2022048689 cites W2097441294 @default.
• W2022048689 cites W2098076093 @default.
• W2022048689 cites W2100738570 @default.
• W2022048689 cites W2150260980 @default.
• W2022048689 cites W2160421139 @default.
• W2022048689 cites W2297407431 @default.
• W2022048689 cites W77187387 @default.
• W2022048689 doi "https://doi.org/10.1042/bj3430385" @default.
• W2022048689 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/1220565" @default.
• W2022048689 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/10510304" @default.
• W2022048689 hasPublicationYear "1999" @default.
• W2022048689 type Work @default.
• W2022048689 sameAs 2022048689 @default.
• W2022048689 citedByCount "13" @default.
• W2022048689 countsByYear W20220486892014 @default.
• W2022048689 countsByYear W20220486892015 @default.
• W2022048689 crossrefType "journal-article" @default.
• W2022048689 hasAuthorship W2022048689A5002044987 @default.
• W2022048689 hasAuthorship W2022048689A5025126230 @default.
• W2022048689 hasAuthorship W2022048689A5033027657 @default.
• W2022048689 hasAuthorship W2022048689A5071187146 @default.
• W2022048689 hasAuthorship W2022048689A5074937251 @default.
• W2022048689 hasBestOaLocation W20220486892 @default.
• W2022048689 hasConcept C121332964 @default.
• W2022048689 hasConcept C178666793 @default.
• W2022048689 hasConcept C181199279 @default.
• W2022048689 hasConcept C181912034 @default.
• W2022048689 hasConcept C185592680 @default.
• W2022048689 hasConcept C18903297 @default.
• W2022048689 hasConcept C2776165026 @default.
• W2022048689 hasConcept C2777289219 @default.
• W2022048689 hasConcept C2778401398 @default.
• W2022048689 hasConcept C2779281246 @default.
• W2022048689 hasConcept C2780340462 @default.
• W2022048689 hasConcept C43617362 @default.
• W2022048689 hasConcept C55493867 @default.
• W2022048689 hasConcept C62520636 @default.
• W2022048689 hasConcept C85528070 @default.
• W2022048689 hasConcept C86803240 @default.
• W2022048689 hasConcept C91881484 @default.
• W2022048689 hasConceptScore W2022048689C121332964 @default.
• W2022048689 hasConceptScore W2022048689C178666793 @default.
• W2022048689 hasConceptScore W2022048689C181199279 @default.
• W2022048689 hasConceptScore W2022048689C181912034 @default.
• W2022048689 hasConceptScore W2022048689C185592680 @default.
• W2022048689 hasConceptScore W2022048689C18903297 @default.
• W2022048689 hasConceptScore W2022048689C2776165026 @default.
• W2022048689 hasConceptScore W2022048689C2777289219 @default.
• W2022048689 hasConceptScore W2022048689C2778401398 @default.
• W2022048689 hasConceptScore W2022048689C2779281246 @default.
• W2022048689 hasConceptScore W2022048689C2780340462 @default.
• W2022048689 hasConceptScore W2022048689C43617362 @default.
• W2022048689 hasConceptScore W2022048689C55493867 @default.
• W2022048689 hasConceptScore W2022048689C62520636 @default.
• W2022048689 hasConceptScore W2022048689C85528070 @default.
• W2022048689 hasConceptScore W2022048689C86803240 @default.
• W2022048689 hasConceptScore W2022048689C91881484 @default.
• W2022048689 hasIssue "2" @default.
• W2022048689 hasLocation W20220486891 @default.
• W2022048689 hasLocation W20220486892 @default.
• W2022048689 hasLocation W20220486893 @default.
• W2022048689 hasOpenAccess W2022048689 @default.
• W2022048689 hasPrimaryLocation W20220486891 @default.
• W2022048689 hasRelatedWork W1552337332 @default.
• W2022048689 hasRelatedWork W1928718700 @default.
• W2022048689 hasRelatedWork W1978210293 @default.
• W2022048689 hasRelatedWork W2020658712 @default.
• W2022048689 hasRelatedWork W2056470407 @default.
• W2022048689 hasRelatedWork W2061891400 @default.
• W2022048689 hasRelatedWork W2065409703 @default.

• next