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• W2022051259 abstract "Cancer and many other diseases are characterized by changes in cell morphology, motion, and mechanical rigidity. However, in live cell cytology, stimulus-induced morphologic changes typically take 10−30 min to detect. Here, we employ live-cell interferometry (LCI) to visualize the rapid response of a whole cell to mechanical stimulation, on a time scale of seconds, and we detect cytoskeletal remodeling behavior within 200 s. This behavior involved small, rapid changes in cell content and miniscule changes in shape; it would be difficult to detect with conventional or phase contrast microscopy alone and is beyond the dynamic capability of AFM. We demonstrate that LCI provides a rapid, quantitative reconstruction of the cell body with no labeling. This is an advantage over traditional microscopy and flow cytometry, which require cell surface tagging and/or destructive cell fixation for labeling" @default.
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• W2022051259 date "2008-05-01" @default.
• W2022051259 modified "2023-09-23" @default.
• W2022051259 title "Live Cell Interferometry Reveals Cellular Dynamism During Force Propagation" @default.
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• W2022051259 doi "https://doi.org/10.1021/nn700303f" @default.
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