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- W2022061711 abstract "Abstract 1. 1. The conjugation of the metabolites of [16- 14 C]estrone in rat liver has been studied utilizing gas-liquid chromatography for the characterization of micro-quantities of estrogen metabolites. 2. 2. The sulfate (13.9%), glucosiduronate (10.3%), and phosphate (4.1%), estrogen conjugates and the unconjugated estrogen metabolites (47.4%) have been isolated from liver slices incubated under air. Incubation under an O 2 atmosphere doubles the sulfate fraction at the expense of the unconjugated estrogens. 3. 3. The phenolic estrogen metabolites in each of these fractions have been analyzed by gas-liquid chromatography. The metabolites of [16- 14 C]estrone (23.6% recovered) that have been characterized are 17β-estradiol (2.2%), estriol (0.97%), 2-methoxyestrone (6.2%), 2-methoxy-17β-estradiol (0.36%). In addition, the presence of three unidentified phenolic metabolites and an acidic component(s) has been 4. 4. Certain of the estrogen metabolites were found in only one conjugated form. For example, the sulfate fraction is the only source of 2-methoxy-17β-estradiol in addition to two unidentified phenolic estrogens. Estrone, on the other hand, is not conjugated in the form of a sulfate. Nearly the entire phosphate fraction consists of an unidentified carboxylic acid metabolite." @default.
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- W2022061711 date "1965-06-01" @default.
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- W2022061711 title "The conjugation of estrogen metabolites by liver slices" @default.
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- W2022061711 doi "https://doi.org/10.1016/0304-4165(65)90242-4" @default.
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