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- W2022070358 abstract "First you must design the primers—PCR! Make sure that they won't form dimers—PCR! Then you check the melting T's A's and T's are two degrees And it's four for G's and C's in PCR! Oh the thermocycler's set for PCR! If this fails you'll be upset with PCR! First you melt and did I mention It's anneal and then extension Copying is the intention—PCR! Many times the protocol you've tried to fix Checking all your tubes and then your mastermix Looking for contamination As you build up your frustration If there's no amplification—PCR! You can use reverse transcription—PCR! Surely you'll throw a conniption—PCR! Other types may give you trouble Oh there's inverse and there's bubble Your anxiety will double—PCR Oh you've really got to amplify this strand Hope that when you run your gel you've got a band Didn't get what you expected Damn this project you selected Now you're feeling quite dejected—PCR! Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article." @default.
- W2022070358 created "2016-06-24" @default.
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- W2022070358 date "2009-05-01" @default.
- W2022070358 modified "2023-09-26" @default.
- W2022070358 title "Song: PCR woes (to the tune of “She'll Be Comin' Round the Mountain”)" @default.
- W2022070358 doi "https://doi.org/10.1002/bmb.20275" @default.
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