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• W2022074166 abstract "This study presents surface-mediated targeted in situ gene delivery from gene-tagged poly(D,L-lactic acid) (PDLLA) films, which were fabricated via a layer-by-layer (LbL) assembly technique with galactosylated polyethylenimine (GP) and plasmid DNA (pDNA, pSV-β-galactosidase). A linear growth of GP/pDNA multilayered films was observed. The pDNA was continuously released from multilayered films for over 32 h. The multilayered structure degraded and simultaneously formed GP/pDNA complexes in situ when exposing to a physiological environment. The pDNA was well protected by GP against DNase I digestion within formed GP/pDNA complexes. Our results demonstrated that GP contributes to receptor-mediated targeting for cell uptake and in situ gene transfection. The results reported here are potentially important for gene therapy, surface engineering of biomaterials, tissue engineering and implant technology." @default.
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• W2022074166 date "2009-05-01" @default.
• W2022074166 modified "2023-09-23" @default.
• W2022074166 title "Fabrication of Galactosylated Polyethylenimine and Plasmid DNA Multilayers on poly (D,L-lactic acid) Films for in situ Targeted Gene Transfection" @default.
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• W2022074166 doi "https://doi.org/10.1002/adem.200800342" @default.
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