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- W2022075083 abstract "Abstract A fibroblast clone SM-Cl was established by cloning cultured fibroblasts derived from a rat carrageenin granuloma. The cloned fibroblasts were found to produce and secrete large amounts of acidic glycosaminoglycans (AGAG) and collagen into the medium, and the AGAG were identified with chondroitin 4-sulfate and hyaluronic acid by 2-dimensional electrophoresis and enzymatic digestion. The cells were exposed to 10 −6 M cortisol or 10 −4 M tetrahydrocortisol for 2 days during their stationary phase. The amounts of AGAG and collagen secreted into the medium, the protein and RNA contents of the cells, the amounts of free amino acids per 10 9 cells, the intracellular concentration of each free amino acid, and the distribution ratio (ratio of intracellular concentration to that in the medium) of each free amino acid of the cells were compared with those of control cells. The production of intracellular substances was markedly inhibited by cortisol, i.e., by 75 per cent for AGAG and by 50 per cent for collagen. Both the amount of each amino acid per 10 9 cells and the distribution ratios were markedly depressed by cortisol treatment for all the determined amino acids except serine. The cloned fibroblasts were then studied with regard to the rate of uptake of 2-amino[1- 14 C]isobutyric acid (AIB) from the medium by control and steroid-treated cells. Cortisol treatment decreased [ 14 C]AIB uptake of the cell markedly from the beginning up to 90 min of incubation. Tetrahydrocortisol, one of the main metabolites of cortisol, exerted no effect even at a concentration as high as 10 −4 M. Incorporation of [ 3 H]thymidine into DNA of the cells in the growing phase was affected very slightly by the drugs." @default.
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- W2022075083 title "Effects of cortisol and tetrahydrocortisol on the cloned fibroblast derived from rat carrageenin granuloma" @default.
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- W2022075083 doi "https://doi.org/10.1016/0006-2952(76)90505-0" @default.
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