FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2022092406> ?p ?o ?g. }

• W2022092406 endingPage "1670" @default.
• W2022092406 startingPage "1665" @default.
• W2022092406 abstract "ObjectiveTo transfect the p53 and p21 gene into the leiomyoma cells isolated from patients and observe their influence on the cell proliferation, leukemia inhibitory factor production, and gene expression of collagen type I as well as tumor necrosis factor-α (TNF-α) of cultured cells.DesignProspective study.SettingAn assisted reproductive technology (ART) and genetic unit of a medical center.Patient(s)Leiomyoma cells isolated from leiomyoma tissue of 12 patients were divided into three groups: [1] vehicle DNA, [2] p53 gene, and [3] p21 gene transfections.Intervention(s)The pcDNA3.1 was used as vector to carry p53 and p21 genes for transfer. After gene transfection, RNAs of the leiomyoma cells were extracted for further analyses of gene expression.Main outcome measure(s)Relative cell numbers were determined by 3-(4,5-dimethylthiazol-2-yl)2,5-diphenyl tetrazolium bromide (MTT) assay. The leukemia inhibitory factor (LIF) concentration was determined with ELISA. Gene expressions of collagen type I and TNF-α were detected by reverse transcriptase-polymerase chain reaction (RT-PCR). Gene expression of glyceraldehyde-3-phosphate dehydrogenase (GAPDH) was used as an internal control. The cell proliferation, LIF production, as well as gene expressions of collagen type I and TNF-α in each group were compared.ResultsRelative cell numbers (%)/LIF production (in picograms per milliliter) in each group were: [1] 100/58, [2] 71/43, and [3] 106/65. The ratios of gene expression of collagen type I/TNF-α with GAPDH in each group were: [1] 1.64/0.335, [2] 1.25/0.434, and [3] 1.77/0.234.Conclusion(s)Transfection with p53 significantly inhibits proliferation of leiomyoma cells and decreases collagen type I gene expression and LIF production. The p21 transfection inhibits TNF-α gene expression." @default.
• W2022092406 created "2016-06-24" @default.
• W2022092406 creator A5046217092 @default.
• W2022092406 creator A5054137637 @default.
• W2022092406 creator A5070182884 @default.
• W2022092406 creator A5072385805 @default.
• W2022092406 date "2004-06-01" @default.
• W2022092406 modified "2023-09-30" @default.
• W2022092406 title "Gene transfections with p53 and p21 inhibit cell proliferation, collagen type I, leukemia inhibitory factor, and tumor necrosis factor-α expression in leiomyoma cells" @default.
• W2022092406 cites W1521027059 @default.
• W2022092406 cites W1559324600 @default.
• W2022092406 cites W1965381596 @default.
• W2022092406 cites W1968232819 @default.
• W2022092406 cites W1972334859 @default.
• W2022092406 cites W2001709285 @default.
• W2022092406 cites W2004050891 @default.
• W2022092406 cites W2014495927 @default.
• W2022092406 cites W2016138321 @default.
• W2022092406 cites W2016471226 @default.
• W2022092406 cites W2016594195 @default.
• W2022092406 cites W2025955532 @default.
• W2022092406 cites W2033584947 @default.
• W2022092406 cites W2033840508 @default.
• W2022092406 cites W2038316001 @default.
• W2022092406 cites W2051695893 @default.
• W2022092406 cites W2059102619 @default.
• W2022092406 cites W2070061120 @default.
• W2022092406 cites W2075176911 @default.
• W2022092406 cites W2084149528 @default.
• W2022092406 cites W2087333277 @default.
• W2022092406 cites W2091421119 @default.
• W2022092406 cites W2092880983 @default.
• W2022092406 cites W2093199751 @default.
• W2022092406 cites W2107891863 @default.
• W2022092406 cites W2117098590 @default.
• W2022092406 cites W2131419766 @default.
• W2022092406 cites W2132286468 @default.
• W2022092406 cites W2132535397 @default.
• W2022092406 cites W2154700137 @default.
• W2022092406 cites W2160547845 @default.
• W2022092406 cites W2163565167 @default.
• W2022092406 cites W2171209024 @default.
• W2022092406 cites W2172800799 @default.
• W2022092406 cites W2315477817 @default.
• W2022092406 cites W2316161621 @default.
• W2022092406 cites W2318856916 @default.
• W2022092406 cites W37723730 @default.
• W2022092406 cites W4214673465 @default.
• W2022092406 cites W4294216491 @default.
• W2022092406 doi "https://doi.org/10.1016/j.fertnstert.2004.02.062" @default.
• W2022092406 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/15193492" @default.
• W2022092406 hasPublicationYear "2004" @default.
• W2022092406 type Work @default.
• W2022092406 sameAs 2022092406 @default.
• W2022092406 citedByCount "6" @default.
• W2022092406 countsByYear W20220924062016 @default.
• W2022092406 countsByYear W20220924062022 @default.
• W2022092406 crossrefType "journal-article" @default.
• W2022092406 hasAuthorship W2022092406A5046217092 @default.
• W2022092406 hasAuthorship W2022092406A5054137637 @default.
• W2022092406 hasAuthorship W2022092406A5070182884 @default.
• W2022092406 hasAuthorship W2022092406A5072385805 @default.
• W2022092406 hasBestOaLocation W20220924061 @default.
• W2022092406 hasConcept C104317684 @default.
• W2022092406 hasConcept C11988809 @default.
• W2022092406 hasConcept C142724271 @default.
• W2022092406 hasConcept C150194340 @default.
• W2022092406 hasConcept C153911025 @default.
• W2022092406 hasConcept C17991360 @default.
• W2022092406 hasConcept C203014093 @default.
• W2022092406 hasConcept C27284151 @default.
• W2022092406 hasConcept C2777795800 @default.
• W2022092406 hasConcept C2778690821 @default.
• W2022092406 hasConcept C502942594 @default.
• W2022092406 hasConcept C54009773 @default.
• W2022092406 hasConcept C54355233 @default.
• W2022092406 hasConcept C55493867 @default.
• W2022092406 hasConcept C62112901 @default.
• W2022092406 hasConcept C71924100 @default.
• W2022092406 hasConcept C81885089 @default.
• W2022092406 hasConcept C86803240 @default.
• W2022092406 hasConceptScore W2022092406C104317684 @default.
• W2022092406 hasConceptScore W2022092406C11988809 @default.
• W2022092406 hasConceptScore W2022092406C142724271 @default.
• W2022092406 hasConceptScore W2022092406C150194340 @default.
• W2022092406 hasConceptScore W2022092406C153911025 @default.
• W2022092406 hasConceptScore W2022092406C17991360 @default.
• W2022092406 hasConceptScore W2022092406C203014093 @default.
• W2022092406 hasConceptScore W2022092406C27284151 @default.
• W2022092406 hasConceptScore W2022092406C2777795800 @default.
• W2022092406 hasConceptScore W2022092406C2778690821 @default.
• W2022092406 hasConceptScore W2022092406C502942594 @default.
• W2022092406 hasConceptScore W2022092406C54009773 @default.
• W2022092406 hasConceptScore W2022092406C54355233 @default.
• W2022092406 hasConceptScore W2022092406C55493867 @default.
• W2022092406 hasConceptScore W2022092406C62112901 @default.
• W2022092406 hasConceptScore W2022092406C71924100 @default.
• W2022092406 hasConceptScore W2022092406C81885089 @default.

• next