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- W2022093051 abstract "To determine the effect of interleukin-10 (IL-10) and transforming growth factor-β (TGF-β) on the down-regulation of MHC class II antigens, bovine retinal pigment epithelial (RPE) cells were incubated with interferon-γ (IFN-γ) in different concentrations. Subsequently, the IFN-γ pretreated RPE cells were cultured with TGF-β or IL-10 in distinct concentrations and treatment modi. About 10% of native (totally untreated) RPE cells were positive for MHC class II antigens detected with immunocytostaining. Under the influence of IFN-γ (1,000 U/ml), the number of MHC class II-bearing cells increased to 49.9 ± 4.5% positive cells after 8 days’ incubation. Expression decreased under the most effective TGF-β treatment (5 ng/ml) to 2.0 ± 0.9% after 24 h incubation, and under similar IL-10 treatment (200 U/ml) to 3.8 ± 1.4% after 72 h. This decreasing number of the MHC class II-positive cells may be useful for various eye research studies and, more especially, to RPE cell transplantation in the future." @default.
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- W2022093051 title "Down-Regulation of MHC Class II Expression on Bovine Retinal Pigment Epithelial Cells by Cytokines" @default.
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