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- W2022101726 abstract "1. The inactivation of the T7 coliphage by HNO 2 is a one-hit process. The inactivation rate is proportional to the HNO 2 concentration and the length of treat ment; it increases when the pH is lowered although, in the absence of HNO 2 , the effect of H + alone is insignificant. 2. DNA interstrand crosslinks explain only a small percentage of the inactivation. The fraction of interstrand crosslinked DNA molecules corresponding to a 63 % inactivation of the phage population is variable and decreases with the time necessary to reach this degree of inactivation; it seems that DNA interstrand crosslinking is a secondary reaction that occurs when the phage is already inactivated. 3. HNO 2 also induces a crosslinkage between the DNA and the coat proteins. This binding which might prevent the injection of the DNA in the host Escherichia coli B can explain only a small percentage of the inactivation hits. 4. Therefore only a small part of the T7 phage inactivation by HNO 2 can be explained by these two types of crosslinkages." @default.
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- W2022101726 title "T7 coliphage inactivation by nitrous acid" @default.
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- W2022101726 doi "https://doi.org/10.1016/0005-2787(70)90039-0" @default.
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