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• W2022112710 abstract "ABSTRACT West Nile virus (WNV) is a neurotropic flavivirus that causes significant neuroinvasive disease involving the brain and/or spinal cord. Experimental mouse models of WNV infection have established the importance of innate and adaptive immune responses in controlling the extent and severity of central nervous system (CNS) disease. However, differentiating between immune responses that are intrinsic to the CNS and those that are dependent on infiltrating inflammatory cells has proven difficult. We used a murine ex vivo spinal cord slice culture (SCSC) model to determine the innate immune processes specific to the CNS during WNV infections. By 7 days after ex vivo infection of SCSCs, the majority of neurons and a substantial percentage of astrocytes were infected with WNV, resulting in apoptotic cell death and astrogliosis. Microglia, the resident immune cells of the CNS, were activated by WNV infection, as exemplified by their amoeboid morphology, the development of filopodia and lamellipodia, and phagocytosis of WNV-infected cells and debris. Microglial cell activation was concomitant with increased expression of proinflammatory cytokines and chemokines, including CXCL10, CXCL1, CCL5, CCL3, CCL2, tumor necrosis factor alpha (TNF-α), TNF-related apoptosis-inducing ligand (TRAIL), and interleukin-6 (IL-6). The application of minocycline, an inhibitor of neuroinflammation, altered the WNV-induced proinflammatory cytokine/chemokine expression profile, with inhibited production of CCL5, CCL2, and IL-6. Our findings establish that CNS-resident cells have the capacity to initiate a robust innate immune response against WNV infection in the absence of infiltrating inflammatory cells and systemic immune responses. IMPORTANCE There are no specific treatments of proven efficacy available for WNV neuroinvasive disease. A better understanding of the pathogenesis of WNV CNS infection is crucial for the rational development of novel therapies. Development of a spinal cord slice culture (SCSC) model facilitates the study of WNV pathogenesis and allows investigation of the intrinsic immune responses of the CNS. Our studies demonstrate that robust CNS innate immune responses, including microglial activation and proinflammatory cytokine/chemokine production, develop independently of contributions from the peripheral immune system and CNS-infiltrating inflammatory cells." @default.
• W2022112710 created "2016-06-24" @default.
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• W2022112710 creator A5011300980 @default.
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• W2022112710 creator A5043820148 @default.
• W2022112710 date "2014-11-15" @default.
• W2022112710 modified "2023-10-10" @default.
• W2022112710 title "Activation of Intrinsic Immune Responses and Microglial Phagocytosis in an <i>Ex Vivo</i> Spinal Cord Slice Culture Model of West Nile Virus Infection" @default.
• W2022112710 cites W1600316672 @default.
• W2022112710 cites W1638564330 @default.
• W2022112710 cites W1963664876 @default.
• W2022112710 cites W1964759127 @default.
• W2022112710 cites W1964963886 @default.
• W2022112710 cites W1967720787 @default.
• W2022112710 cites W1969696108 @default.
• W2022112710 cites W1972326511 @default.
• W2022112710 cites W1973308848 @default.
• W2022112710 cites W1975919336 @default.
• W2022112710 cites W1980647681 @default.
• W2022112710 cites W1984760454 @default.
• W2022112710 cites W1987778810 @default.
• W2022112710 cites W1994581225 @default.
• W2022112710 cites W1995365045 @default.
• W2022112710 cites W1999494818 @default.
• W2022112710 cites W1999732999 @default.
• W2022112710 cites W2003438800 @default.
• W2022112710 cites W2004492484 @default.
• W2022112710 cites W2006644445 @default.
• W2022112710 cites W2009998721 @default.
• W2022112710 cites W2011142778 @default.
• W2022112710 cites W2013790112 @default.
• W2022112710 cites W2017756051 @default.
• W2022112710 cites W2018722698 @default.
• W2022112710 cites W2030448361 @default.
• W2022112710 cites W2037466974 @default.
• W2022112710 cites W2043361020 @default.
• W2022112710 cites W2043622101 @default.
• W2022112710 cites W2045773333 @default.
• W2022112710 cites W2049285992 @default.
• W2022112710 cites W2051329301 @default.
• W2022112710 cites W2053176712 @default.
• W2022112710 cites W2055651600 @default.
• W2022112710 cites W2057310829 @default.
• W2022112710 cites W2059369279 @default.
• W2022112710 cites W2063990167 @default.
• W2022112710 cites W2063995449 @default.
• W2022112710 cites W2067267561 @default.
• W2022112710 cites W2067499927 @default.
• W2022112710 cites W2073615145 @default.
• W2022112710 cites W2073615594 @default.
• W2022112710 cites W2081654862 @default.
• W2022112710 cites W2084603039 @default.
• W2022112710 cites W2088183665 @default.
• W2022112710 cites W2093305459 @default.
• W2022112710 cites W2095241201 @default.
• W2022112710 cites W2095291159 @default.
• W2022112710 cites W2095767844 @default.
• W2022112710 cites W2096954367 @default.
• W2022112710 cites W2102947626 @default.
• W2022112710 cites W2103627231 @default.
• W2022112710 cites W2105288008 @default.
• W2022112710 cites W2107959693 @default.
• W2022112710 cites W2113507178 @default.
• W2022112710 cites W2115296748 @default.
• W2022112710 cites W2119958966 @default.
• W2022112710 cites W2120996101 @default.
• W2022112710 cites W2123877754 @default.
• W2022112710 cites W2124078703 @default.
• W2022112710 cites W2133961780 @default.
• W2022112710 cites W2136123169 @default.
• W2022112710 cites W2138778124 @default.
• W2022112710 cites W2142913420 @default.
• W2022112710 cites W2149538964 @default.
• W2022112710 cites W2156131771 @default.
• W2022112710 cites W2156844153 @default.
• W2022112710 cites W2163751922 @default.
• W2022112710 cites W2164191009 @default.
• W2022112710 cites W2164255537 @default.
• W2022112710 cites W2171534203 @default.
• W2022112710 cites W2171549018 @default.
• W2022112710 doi "https://doi.org/10.1128/jvi.01994-14" @default.
• W2022112710 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4249089" @default.
• W2022112710 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/25165111" @default.
• W2022112710 hasPublicationYear "2014" @default.
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