Matches in SemOpenAlex for { <https://semopenalex.org/work/W2022115757> ?p ?o ?g. }
- W2022115757 endingPage "615" @default.
- W2022115757 startingPage "615" @default.
- W2022115757 abstract "The production of transgenic farm animals will be greatly enhanced with the development of cultured cell lines that remain totipotent following nuclear transfer. Here, data are presented that demonstrate the generation of both male and female cloned lambs from two established embryonic cell lines. Cytoplasts derived from in vivo oocytes resulted in slightly greater development to blastocyst (24% v. 17%) and survival to term (7% v. 2%) compared with in vitro oocytes. There was no advantage in co-culturing cloned embryos with oviductal epithelial cells compared with synthetic oviductal fluid medium in terms of development to blastocyst (18% v. 31%) or survival to term (both 8%). Although the survival of cloned embryos immediately after transfer was high based on 'biochemical' pregnancy, 64-80% of embryos failed over the attachment phase with in vivo cytoplasts. Although the co-transfer of trophoblastic vesicles improved embryo survival to Day 35 (45% v. 25%), there was no difference at term. A high proportion of fetuses were lost during the last trimester (43%), resulting in 11% of embryos transferred developing to term using in vivo cytoplasts (12/112). Five lambs have survived and two rams are fertile. The current nuclear transfer process is inefficient and further research is needed to improve the development of healthy fetuses." @default.
- W2022115757 created "2016-06-24" @default.
- W2022115757 creator A5003071473 @default.
- W2022115757 creator A5010739973 @default.
- W2022115757 creator A5024502250 @default.
- W2022115757 creator A5058126192 @default.
- W2022115757 creator A5087851290 @default.
- W2022115757 date "1998-01-01" @default.
- W2022115757 modified "2023-09-26" @default.
- W2022115757 title "Cloning sheep from cultured embryonic cells" @default.
- W2022115757 cites W135451167 @default.
- W2022115757 cites W154576131 @default.
- W2022115757 cites W1967605947 @default.
- W2022115757 cites W1969523318 @default.
- W2022115757 cites W1970868451 @default.
- W2022115757 cites W1970894097 @default.
- W2022115757 cites W1973992851 @default.
- W2022115757 cites W1982456152 @default.
- W2022115757 cites W1992437424 @default.
- W2022115757 cites W1992525863 @default.
- W2022115757 cites W1998575393 @default.
- W2022115757 cites W2000778717 @default.
- W2022115757 cites W2002116819 @default.
- W2022115757 cites W2003701368 @default.
- W2022115757 cites W2009609926 @default.
- W2022115757 cites W2010955459 @default.
- W2022115757 cites W2012096564 @default.
- W2022115757 cites W2020218620 @default.
- W2022115757 cites W2022691143 @default.
- W2022115757 cites W2030583225 @default.
- W2022115757 cites W2031148277 @default.
- W2022115757 cites W2035247129 @default.
- W2022115757 cites W2040855835 @default.
- W2022115757 cites W2045899482 @default.
- W2022115757 cites W2049987070 @default.
- W2022115757 cites W2051786711 @default.
- W2022115757 cites W2059727398 @default.
- W2022115757 cites W2062920544 @default.
- W2022115757 cites W2072600684 @default.
- W2022115757 cites W2075679174 @default.
- W2022115757 cites W2079611048 @default.
- W2022115757 cites W2085360111 @default.
- W2022115757 cites W2089443863 @default.
- W2022115757 cites W2089805173 @default.
- W2022115757 cites W2089959711 @default.
- W2022115757 cites W2097236698 @default.
- W2022115757 cites W2116978641 @default.
- W2022115757 cites W2127442070 @default.
- W2022115757 cites W2139789898 @default.
- W2022115757 cites W2140338297 @default.
- W2022115757 cites W2152535373 @default.
- W2022115757 cites W2157901179 @default.
- W2022115757 cites W2170484225 @default.
- W2022115757 cites W2171892478 @default.
- W2022115757 cites W2240161071 @default.
- W2022115757 cites W2327546452 @default.
- W2022115757 cites W2416064565 @default.
- W2022115757 cites W575693168 @default.
- W2022115757 doi "https://doi.org/10.1071/rd98046" @default.
- W2022115757 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/10612467" @default.
- W2022115757 hasPublicationYear "1998" @default.
- W2022115757 type Work @default.
- W2022115757 sameAs 2022115757 @default.
- W2022115757 citedByCount "29" @default.
- W2022115757 countsByYear W20221157572018 @default.
- W2022115757 countsByYear W20221157572019 @default.
- W2022115757 countsByYear W20221157572020 @default.
- W2022115757 countsByYear W20221157572022 @default.
- W2022115757 crossrefType "journal-article" @default.
- W2022115757 hasAuthorship W2022115757A5003071473 @default.
- W2022115757 hasAuthorship W2022115757A5010739973 @default.
- W2022115757 hasAuthorship W2022115757A5024502250 @default.
- W2022115757 hasAuthorship W2022115757A5058126192 @default.
- W2022115757 hasAuthorship W2022115757A5087851290 @default.
- W2022115757 hasConcept C104317684 @default.
- W2022115757 hasConcept C121050878 @default.
- W2022115757 hasConcept C145103041 @default.
- W2022115757 hasConcept C160099875 @default.
- W2022115757 hasConcept C16685009 @default.
- W2022115757 hasConcept C172680121 @default.
- W2022115757 hasConcept C190546358 @default.
- W2022115757 hasConcept C196843134 @default.
- W2022115757 hasConcept C199360897 @default.
- W2022115757 hasConcept C203014093 @default.
- W2022115757 hasConcept C207001950 @default.
- W2022115757 hasConcept C2778177303 @default.
- W2022115757 hasConcept C2779234561 @default.
- W2022115757 hasConcept C2910790502 @default.
- W2022115757 hasConcept C41008148 @default.
- W2022115757 hasConcept C54355233 @default.
- W2022115757 hasConcept C71924100 @default.
- W2022115757 hasConcept C86803240 @default.
- W2022115757 hasConcept C87073359 @default.
- W2022115757 hasConcept C95444343 @default.
- W2022115757 hasConceptScore W2022115757C104317684 @default.
- W2022115757 hasConceptScore W2022115757C121050878 @default.
- W2022115757 hasConceptScore W2022115757C145103041 @default.
- W2022115757 hasConceptScore W2022115757C160099875 @default.