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• W2022117557 abstract "Chitosan-based nanoparticles (chiNPs) are considered to be potentially good carriers for the sustained intracellular delivery of specific molecules. However, scarce attention has been paid to the long-lasting permanence of these NPs in the intracellular milieu, as well as to their intracellular fate (i.e., distribution, interaction with cell organelles, and degradation) in the long term. In the present study, the presence and subcellular location of FITC-labelled chiNPs were monitored in HeLa cells up to 14 days post-administration using multicolor-fluorescence confocal microscopy and diaminobenzidine photo-oxidation at transmission electron microscopy. The main result of the present study is the demonstration that internalized chiNPs persist inside the cell up to two weeks, occurring in both the cytoplasm and nucleus; accordingly, chiNPs are able to pass from mother to daughter cells through several mitotic cycles. The cells did not show increased mortality or structural damage up to 14 days after chiNP exposure." @default.
• W2022117557 created "2016-06-24" @default.
• W2022117557 creator A5006434756 @default.
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• W2022117557 date "2015-02-18" @default.
• W2022117557 modified "2023-09-26" @default.
• W2022117557 title "Internalized chitosan nanoparticles persist for long time in cultured cells" @default.
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• W2022117557 doi "https://doi.org/10.4081/ejh.2015.2492" @default.
• W2022117557 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4378219" @default.
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