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- W2022120625 abstract "This investigation was aimed at the development of a photometric system capable of measuring hematocrit in glass capillary tubes (37 to 116 μm i.d.). Such an in vitro study would provide the calibration needed for in vivo measurements of microvascular hematocrit in arterioles and venules where the red blood cells are not discernible. In the experimental setup, a tube was positioned on the stage of a transmission light microscope. The magnified image of the tube was cast on a viewing screen. By aligning a fiber optic with the image, the transmitted light was directed to a photomultiplier module and the response was recorded. The optical density was calculated from the ratio of light intensity for a given hematocrit and that for the plasma filled tube. With the aid of a parametric analysis, a series of preliminary experiments demonstrated that the flow rate, the input level of the light intensity, oxygen saturation, and hemoglobin content had a minimal effect on the optical density for the four sizes of capillary used. (The effect of flow rate is not negligible for tubes larger than 190 μm.) The unfiltered light and the 405 and 560 μm monochromatic light were used as the transmission light. It was found that the unfiltered light was most convenient to use and provide the best resolution at high hematocrit. Since the measured optical density is shown to be the same for 450 and 560 μm monochromatic light and the hemoglobin absorption at the former wavelength is about 10 times that of the latter, the light loss through the capillary tube is primarily due to light scattering by the red blood cells. The important independent quantities which determined the optical density were the tube hematocrit and the ratio of tube diameter to erythrocyte diameter. For each tube size, a curve-relating optical density to tube hematocrit was presented. Although the curve was similar to one-half of a parabola, sufficient resolution was obtained for the measurement of an unknown hematocrit in the physiological range." @default.
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- W2022120625 date "1973-11-01" @default.
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- W2022120625 title "The measurement of hematocrit of blood flowing in glass capillaries by microphotometry" @default.
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- W2022120625 doi "https://doi.org/10.1016/0026-2862(73)90080-0" @default.
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