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- W2022128375 abstract "Testis sheaths and fat body from developing male pupae of Heliothis virescens synthesize soluble growth factor-like products when exposed to 20-hydroxyecdysone (20HE). These factors promote growth and development of the genital tract. Saline extracts of modified Grace's medium and 20HE-treated testis sheaths and fat body were subjected to heat, freeze-thaw, organic solvents, and low pressure size exclusion chromatography. Although extracts were stable to repeated freeze-thawing, activity was lost after exposure to organic solvents; activities of fractions heavier than 6.5 KDa were inhibited by heating to 100°C. Size exclusion chromatography yielded 10 active testis extract fractions and 9 active fat body fractions. Although the approximate molecular weights of most of the extract fractions were similar, enzyme studies using protease, lipase, and α-amylase indicated differences in the chemistry of active fractions derived from the two tissues. Active factors were inhibited by protease or lipase or both enzymes. © 1994 Wiley-Liss, Inc." @default.
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- W2022128375 date "1994-01-01" @default.
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- W2022128375 title "Characterization of genital tract growth factor-like activity from testis sheaths and fat body ofHeliothis virescens" @default.
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- W2022128375 doi "https://doi.org/10.1002/arch.940260403" @default.
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