Matches in SemOpenAlex for { <https://semopenalex.org/work/W2022129227> ?p ?o ?g. }
Showing items 1 to 92 of
92
with 100 items per page.
- W2022129227 endingPage "2344" @default.
- W2022129227 startingPage "2341" @default.
- W2022129227 abstract "Although the alternative splicing of various genes is a common event in human tumors, the mechanisms behind it have not been characterized. We hypothesized that the expression of splicing regulatory factors would be changed during cellular transformation. Gene expression of three splicing regulatory factors, alternative splicing factor/splicing factor 2 (ASF/SF2), heterogeneous nuclear ribonucleoprotein A2 (hnRNP A2) and the 65 kDa subunit of U2 small nuclear ribonucleoprotein particles auxiliary factor (U2AF(65)), were examined by northern blotting in a two-step chemical transformation model. This in vitro model is composed of BALB/3T3 cells and a BALB/3T3-derived N-methyl-N-nitro-N-nitrosoguanidine (MNNG)-initiated cell line (MT-5). MT-5 cells can be transformed on exposure to 12-O-tetradecanoylphorbol-13-acetate (TPA). ASF/SF2 mRNA levels were decreased 2-fold in both MNNG-initiated cells and TPA-induced transformed cells compared with the normal parental cells, whereas hnRNP A2 mRNA expression did not significantly change between these three types of cells. U2AF(65) mRNA levels were markedly increased ( approximately 4.7-fold) associated with progression of cellular transformation. Moreover, RT-PCR analysis showed that distinct forms of ASF/SF2 mRNA were present in the MNNG-initiated cells and TPA-induced transformed cells but not in the parental cells. These findings indicate that ASF/SF2 or U2AF(65) gene expression is altered during in vitro two-step chemical transformation. The data suggest that the differential expression of splicing regulatory factors is one cause of aberrant expression of alternatively spliced mRNAs encoded by various genes in tumor cells." @default.
- W2022129227 created "2016-06-24" @default.
- W2022129227 creator A5034628275 @default.
- W2022129227 creator A5079774591 @default.
- W2022129227 creator A5090276104 @default.
- W2022129227 date "1999-12-01" @default.
- W2022129227 modified "2023-09-26" @default.
- W2022129227 title "Differential expression of the splicing regulatory factor genes during two-step chemical transformation in a BALB/3T3-derived cell line, MT-5" @default.
- W2022129227 cites W1555039620 @default.
- W2022129227 cites W1647720439 @default.
- W2022129227 cites W1969418387 @default.
- W2022129227 cites W1973916539 @default.
- W2022129227 cites W1975580175 @default.
- W2022129227 cites W1976010818 @default.
- W2022129227 cites W1999767229 @default.
- W2022129227 cites W2016505381 @default.
- W2022129227 cites W2022647519 @default.
- W2022129227 cites W2027593556 @default.
- W2022129227 cites W2049847998 @default.
- W2022129227 cites W2063225252 @default.
- W2022129227 cites W2071336621 @default.
- W2022129227 cites W2071589653 @default.
- W2022129227 cites W2082127202 @default.
- W2022129227 cites W2116884417 @default.
- W2022129227 cites W2117976997 @default.
- W2022129227 cites W2124947814 @default.
- W2022129227 cites W2131249604 @default.
- W2022129227 cites W2170255408 @default.
- W2022129227 cites W235098460 @default.
- W2022129227 cites W268783301 @default.
- W2022129227 doi "https://doi.org/10.1093/carcin/20.12.2341" @default.
- W2022129227 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/10590230" @default.
- W2022129227 hasPublicationYear "1999" @default.
- W2022129227 type Work @default.
- W2022129227 sameAs 2022129227 @default.
- W2022129227 citedByCount "10" @default.
- W2022129227 crossrefType "journal-article" @default.
- W2022129227 hasAuthorship W2022129227A5034628275 @default.
- W2022129227 hasAuthorship W2022129227A5079774591 @default.
- W2022129227 hasAuthorship W2022129227A5090276104 @default.
- W2022129227 hasConcept C104317684 @default.
- W2022129227 hasConcept C105580179 @default.
- W2022129227 hasConcept C150194340 @default.
- W2022129227 hasConcept C153911025 @default.
- W2022129227 hasConcept C194583182 @default.
- W2022129227 hasConcept C2908584439 @default.
- W2022129227 hasConcept C2909472194 @default.
- W2022129227 hasConcept C54355233 @default.
- W2022129227 hasConcept C54458228 @default.
- W2022129227 hasConcept C67705224 @default.
- W2022129227 hasConcept C73758832 @default.
- W2022129227 hasConcept C75934600 @default.
- W2022129227 hasConcept C81885089 @default.
- W2022129227 hasConcept C86803240 @default.
- W2022129227 hasConcept C95444343 @default.
- W2022129227 hasConceptScore W2022129227C104317684 @default.
- W2022129227 hasConceptScore W2022129227C105580179 @default.
- W2022129227 hasConceptScore W2022129227C150194340 @default.
- W2022129227 hasConceptScore W2022129227C153911025 @default.
- W2022129227 hasConceptScore W2022129227C194583182 @default.
- W2022129227 hasConceptScore W2022129227C2908584439 @default.
- W2022129227 hasConceptScore W2022129227C2909472194 @default.
- W2022129227 hasConceptScore W2022129227C54355233 @default.
- W2022129227 hasConceptScore W2022129227C54458228 @default.
- W2022129227 hasConceptScore W2022129227C67705224 @default.
- W2022129227 hasConceptScore W2022129227C73758832 @default.
- W2022129227 hasConceptScore W2022129227C75934600 @default.
- W2022129227 hasConceptScore W2022129227C81885089 @default.
- W2022129227 hasConceptScore W2022129227C86803240 @default.
- W2022129227 hasConceptScore W2022129227C95444343 @default.
- W2022129227 hasIssue "12" @default.
- W2022129227 hasLocation W20221292271 @default.
- W2022129227 hasLocation W20221292272 @default.
- W2022129227 hasOpenAccess W2022129227 @default.
- W2022129227 hasPrimaryLocation W20221292271 @default.
- W2022129227 hasRelatedWork W1500888016 @default.
- W2022129227 hasRelatedWork W1502076562 @default.
- W2022129227 hasRelatedWork W1973916539 @default.
- W2022129227 hasRelatedWork W2052498125 @default.
- W2022129227 hasRelatedWork W2068816187 @default.
- W2022129227 hasRelatedWork W2082127202 @default.
- W2022129227 hasRelatedWork W2094867951 @default.
- W2022129227 hasRelatedWork W2116403076 @default.
- W2022129227 hasRelatedWork W2151078655 @default.
- W2022129227 hasRelatedWork W2968927049 @default.
- W2022129227 hasVolume "20" @default.
- W2022129227 isParatext "false" @default.
- W2022129227 isRetracted "false" @default.
- W2022129227 magId "2022129227" @default.
- W2022129227 workType "article" @default.