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- W2022131186 abstract "In plant systems, the green fluorescent protein (GFP) is increasingly used as a marker to study dynamics of the secretory apparatus using fluorescence microscopy. The purpose of this study was to immunogold localize the GFP, at the electron microscopic level, in a line of tobacco BY-2-cultured cells, expressing a GFP-tagged Golgi glycosyltransferase. To this end we have developed a simple, one-step chemical fixation method that allow good structural preservation and specific labeling with anti-GFP antibodies. Using this method, we have been able to show that an N-glycan GFP-tagged xylosyltransferase is specifically associated with Golgi stacks of BY-2 transformed cells and is preferentially located in medial cisternae. As an alternative to cryofixation methods, such as high-pressure freezing, which requires specialized and expensive equipment not available in most laboratories, this method offers researchers the opportunity to investigate GFP-tagged proteins of the endomembrane system in tobacco BY-2 cells." @default.
- W2022131186 created "2016-06-24" @default.
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- W2022131186 date "2003-07-01" @default.
- W2022131186 modified "2023-10-13" @default.
- W2022131186 title "An Improved Chemical Fixation Method Suitable for Immunogold Localization of Green Fluorescent Protein in the Golgi Apparatus of Tobacco Bright Yellow (BY-2) Cells" @default.
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- W2022131186 doi "https://doi.org/10.1177/002215540305100708" @default.
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