Matches in SemOpenAlex for { <https://semopenalex.org/work/W2022131193> ?p ?o ?g. }
Showing items 1 to 91 of
91
with 100 items per page.
- W2022131193 endingPage "258" @default.
- W2022131193 startingPage "255" @default.
- W2022131193 abstract "The gender of cercarial larvae can only be determined using molecular methods. End point PCR methods that amplify repetitive markers on the W chromosome of the female (ZW) parasites have been developed, but sometimes results are ambiguous or incorrect. To more effectively distinguish sexes, and to determine why end point PCR can be incorrect, we quantified the W6 repeat sequence and a specific Z chromosome gene using real-time PCR. The ratio between copy number of W6 and a Z chromosome marker unambiguously identifies gender: females have higher ratios (421–4371) than males (0–21). However, some males have low numbers of W6 elements in their genome, and qPCR demonstrated significantly higher W6/Z marker ratios for male genotypes giving ambiguous end point PCR results compared with males giving clear end point results. The quantitative PCR sexing method developed will be particularly useful where reliable sexing of cercariae is critical, for example when staging genetic crosses." @default.
- W2022131193 created "2016-06-24" @default.
- W2022131193 creator A5029844155 @default.
- W2022131193 creator A5053112911 @default.
- W2022131193 creator A5085039675 @default.
- W2022131193 date "1991-08-01" @default.
- W2022131193 modified "2023-09-25" @default.
- W2022131193 title "Sexing single larval stages of Schistosoma mansoni by polymerase chain reaction" @default.
- W2022131193 cites W1624488584 @default.
- W2022131193 cites W1985424839 @default.
- W2022131193 cites W1996236260 @default.
- W2022131193 cites W2005757275 @default.
- W2022131193 cites W2017544701 @default.
- W2022131193 cites W2029429556 @default.
- W2022131193 cites W2032118018 @default.
- W2022131193 cites W2047480444 @default.
- W2022131193 cites W2067024021 @default.
- W2022131193 cites W2075273788 @default.
- W2022131193 cites W2078228619 @default.
- W2022131193 cites W2123986389 @default.
- W2022131193 cites W4301951131 @default.
- W2022131193 doi "https://doi.org/10.1016/0166-6851(91)90187-b" @default.
- W2022131193 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/1944422" @default.
- W2022131193 hasPublicationYear "1991" @default.
- W2022131193 type Work @default.
- W2022131193 sameAs 2022131193 @default.
- W2022131193 citedByCount "43" @default.
- W2022131193 countsByYear W20221311932012 @default.
- W2022131193 countsByYear W20221311932014 @default.
- W2022131193 countsByYear W20221311932016 @default.
- W2022131193 countsByYear W20221311932017 @default.
- W2022131193 countsByYear W20221311932018 @default.
- W2022131193 countsByYear W20221311932019 @default.
- W2022131193 countsByYear W20221311932020 @default.
- W2022131193 countsByYear W20221311932021 @default.
- W2022131193 countsByYear W20221311932022 @default.
- W2022131193 countsByYear W20221311932023 @default.
- W2022131193 crossrefType "journal-article" @default.
- W2022131193 hasAuthorship W2022131193A5029844155 @default.
- W2022131193 hasAuthorship W2022131193A5053112911 @default.
- W2022131193 hasAuthorship W2022131193A5085039675 @default.
- W2022131193 hasConcept C104317684 @default.
- W2022131193 hasConcept C135763542 @default.
- W2022131193 hasConcept C153911025 @default.
- W2022131193 hasConcept C165901193 @default.
- W2022131193 hasConcept C190789776 @default.
- W2022131193 hasConcept C2776225656 @default.
- W2022131193 hasConcept C2776863245 @default.
- W2022131193 hasConcept C2779383911 @default.
- W2022131193 hasConcept C2780246931 @default.
- W2022131193 hasConcept C30481170 @default.
- W2022131193 hasConcept C49105822 @default.
- W2022131193 hasConcept C54355233 @default.
- W2022131193 hasConcept C86803240 @default.
- W2022131193 hasConcept C90856448 @default.
- W2022131193 hasConceptScore W2022131193C104317684 @default.
- W2022131193 hasConceptScore W2022131193C135763542 @default.
- W2022131193 hasConceptScore W2022131193C153911025 @default.
- W2022131193 hasConceptScore W2022131193C165901193 @default.
- W2022131193 hasConceptScore W2022131193C190789776 @default.
- W2022131193 hasConceptScore W2022131193C2776225656 @default.
- W2022131193 hasConceptScore W2022131193C2776863245 @default.
- W2022131193 hasConceptScore W2022131193C2779383911 @default.
- W2022131193 hasConceptScore W2022131193C2780246931 @default.
- W2022131193 hasConceptScore W2022131193C30481170 @default.
- W2022131193 hasConceptScore W2022131193C49105822 @default.
- W2022131193 hasConceptScore W2022131193C54355233 @default.
- W2022131193 hasConceptScore W2022131193C86803240 @default.
- W2022131193 hasConceptScore W2022131193C90856448 @default.
- W2022131193 hasIssue "2" @default.
- W2022131193 hasLocation W20221311931 @default.
- W2022131193 hasLocation W20221311932 @default.
- W2022131193 hasOpenAccess W2022131193 @default.
- W2022131193 hasPrimaryLocation W20221311931 @default.
- W2022131193 hasRelatedWork W1525091092 @default.
- W2022131193 hasRelatedWork W1866648957 @default.
- W2022131193 hasRelatedWork W2008877636 @default.
- W2022131193 hasRelatedWork W2022131193 @default.
- W2022131193 hasRelatedWork W2031436818 @default.
- W2022131193 hasRelatedWork W2035450117 @default.
- W2022131193 hasRelatedWork W2110960267 @default.
- W2022131193 hasRelatedWork W2264094899 @default.
- W2022131193 hasRelatedWork W2318511135 @default.
- W2022131193 hasRelatedWork W2397903800 @default.
- W2022131193 hasVolume "47" @default.
- W2022131193 isParatext "false" @default.
- W2022131193 isRetracted "false" @default.
- W2022131193 magId "2022131193" @default.
- W2022131193 workType "article" @default.