FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2022131447> ?p ?o ?g. }

• W2022131447 endingPage "141" @default.
• W2022131447 startingPage "135" @default.
• W2022131447 abstract "Dermatophilus congolensis is the pathogenic actinomycete that causes dermatophilosis in cattle, lumpy wool in sheep and rain scald in horses. Phenotypic variation between isolates has previously been described, but its genetic basis, extent and importance have not been investigated. Standard DNA extraction methods are not always successful for D. congolensis due to its complex life cycle, one stage of which is encapsulated. Here we describe the development of rapid and reliable DNA extraction and random amplified polymorphic DNA (RAPD) methods that can be used for genotyping D. congolensis field isolates. Our results suggest that genotypic variation between isolates correlates with host species. Several DNA extraction methods and RAPD protocols were compared. An extraction method based on incubation of the bacterium in lysozyme, sodium dodecyl sulphate (SDS) and proteinase K treatments and phenolic extraction yielded high‐quality DNA, which was used to optimize RAPD–polymerase chain reaction (PCR) protocols for two random primers. An alternative rapid, non‐phenolic extraction method based on proteinase K treatment and thermal shock was selected for routine RAPD typing of isolates. DNA extracted from reference strains from cattle, sheep and horse using either method gave reproducible banding patterns with different DNA batches and different thermal cyclers. The rapid DNA extraction method and RAPD–PCR were applied to 38 D. congolensis field isolates. The band patterns of the field and type isolates correlated with host species but not with geographical location." @default.
• W2022131447 created "2016-06-24" @default.
• W2022131447 creator A5022959574 @default.
• W2022131447 creator A5027454694 @default.
• W2022131447 creator A5030128479 @default.
• W2022131447 creator A5040969153 @default.
• W2022131447 creator A5057347791 @default.
• W2022131447 creator A5066689161 @default.
• W2022131447 creator A5071145091 @default.
• W2022131447 creator A5085568244 @default.
• W2022131447 creator A5088186205 @default.
• W2022131447 date "2002-04-01" @default.
• W2022131447 modified "2023-10-15" @default.
• W2022131447 title "A Simple Random Amplified Polymorphic DNA Genotyping Method for Field Isolates of Dermatophilus congolensis" @default.
• W2022131447 cites W1964271797 @default.
• W2022131447 cites W1969540209 @default.
• W2022131447 cites W1973677539 @default.
• W2022131447 cites W1981611524 @default.
• W2022131447 cites W1987438668 @default.
• W2022131447 cites W1993287708 @default.
• W2022131447 cites W1995938887 @default.
• W2022131447 cites W2023589640 @default.
• W2022131447 cites W2030320240 @default.
• W2022131447 cites W2043395203 @default.
• W2022131447 cites W2046027132 @default.
• W2022131447 cites W2047929378 @default.
• W2022131447 cites W2048590527 @default.
• W2022131447 cites W2061853296 @default.
• W2022131447 cites W2068194548 @default.
• W2022131447 cites W2108385864 @default.
• W2022131447 cites W2150438602 @default.
• W2022131447 doi "https://doi.org/10.1046/j.1439-0450.2002.00521.x" @default.
• W2022131447 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/12019944" @default.
• W2022131447 hasPublicationYear "2002" @default.
• W2022131447 type Work @default.
• W2022131447 sameAs 2022131447 @default.
• W2022131447 citedByCount "17" @default.
• W2022131447 countsByYear W20221314472012 @default.
• W2022131447 countsByYear W20221314472013 @default.
• W2022131447 countsByYear W20221314472014 @default.
• W2022131447 countsByYear W20221314472015 @default.
• W2022131447 countsByYear W20221314472016 @default.
• W2022131447 countsByYear W20221314472021 @default.
• W2022131447 countsByYear W20221314472022 @default.
• W2022131447 crossrefType "journal-article" @default.
• W2022131447 hasAuthorship W2022131447A5022959574 @default.
• W2022131447 hasAuthorship W2022131447A5027454694 @default.
• W2022131447 hasAuthorship W2022131447A5030128479 @default.
• W2022131447 hasAuthorship W2022131447A5040969153 @default.
• W2022131447 hasAuthorship W2022131447A5057347791 @default.
• W2022131447 hasAuthorship W2022131447A5066689161 @default.
• W2022131447 hasAuthorship W2022131447A5071145091 @default.
• W2022131447 hasAuthorship W2022131447A5085568244 @default.
• W2022131447 hasAuthorship W2022131447A5088186205 @default.
• W2022131447 hasConcept C104317684 @default.
• W2022131447 hasConcept C135763542 @default.
• W2022131447 hasConcept C144024400 @default.
• W2022131447 hasConcept C149923435 @default.
• W2022131447 hasConcept C153911025 @default.
• W2022131447 hasConcept C2778471508 @default.
• W2022131447 hasConcept C2781209916 @default.
• W2022131447 hasConcept C2908647359 @default.
• W2022131447 hasConcept C31467283 @default.
• W2022131447 hasConcept C42972112 @default.
• W2022131447 hasConcept C49105822 @default.
• W2022131447 hasConcept C54355233 @default.
• W2022131447 hasConcept C552990157 @default.
• W2022131447 hasConcept C71924100 @default.
• W2022131447 hasConcept C78063203 @default.
• W2022131447 hasConcept C79386861 @default.
• W2022131447 hasConcept C81977670 @default.
• W2022131447 hasConcept C84623179 @default.
• W2022131447 hasConcept C86803240 @default.
• W2022131447 hasConcept C89423630 @default.
• W2022131447 hasConceptScore W2022131447C104317684 @default.
• W2022131447 hasConceptScore W2022131447C135763542 @default.
• W2022131447 hasConceptScore W2022131447C144024400 @default.
• W2022131447 hasConceptScore W2022131447C149923435 @default.
• W2022131447 hasConceptScore W2022131447C153911025 @default.
• W2022131447 hasConceptScore W2022131447C2778471508 @default.
• W2022131447 hasConceptScore W2022131447C2781209916 @default.
• W2022131447 hasConceptScore W2022131447C2908647359 @default.
• W2022131447 hasConceptScore W2022131447C31467283 @default.
• W2022131447 hasConceptScore W2022131447C42972112 @default.
• W2022131447 hasConceptScore W2022131447C49105822 @default.
• W2022131447 hasConceptScore W2022131447C54355233 @default.
• W2022131447 hasConceptScore W2022131447C552990157 @default.
• W2022131447 hasConceptScore W2022131447C71924100 @default.
• W2022131447 hasConceptScore W2022131447C78063203 @default.
• W2022131447 hasConceptScore W2022131447C79386861 @default.
• W2022131447 hasConceptScore W2022131447C81977670 @default.
• W2022131447 hasConceptScore W2022131447C84623179 @default.
• W2022131447 hasConceptScore W2022131447C86803240 @default.
• W2022131447 hasConceptScore W2022131447C89423630 @default.
• W2022131447 hasIssue "3" @default.
• W2022131447 hasLocation W20221314471 @default.
• W2022131447 hasLocation W20221314472 @default.
• W2022131447 hasOpenAccess W2022131447 @default.

• next